摘要
对山西省晋中市太谷区的山丹进行深入调查,首次明确了太谷区山丹的分布情况和种质多样性,发现了5种颜色的山丹,并从表型、细胞学、生理学以及分子水平对太谷区野生山丹花色变异的遗传基础与分子机制进行了初步研究。结果表明,太谷区山丹分布在112°39′50″~112°95′64″E、37°19′12″~37°42′17″N、海拔963~1430 m的范围内;除小白乡未发现山丹外,阳邑乡发现683株,范村镇和侯城乡分别发现394株和246株,共1323株。山丹的表型多样性主要表现在株高、茎色、花色、花蕾绒毛及种子颜色等方面。株高的多样性与海拔高度有显著相关性,花色与茎秆颜色的多样性与植物分布的位置有显著相关性。利用比色卡和色差仪,确定了5种山丹花色包括橙色、橙红色、鲜红色、深红、紫红色,其中橙红色最多,占比90.2%。阳邑乡小店村是唯一1个5种花色山丹共存的居群。5种花色色彩差距明显,橙色与橙红色山丹最为鲜艳。类胡萝卜素是山丹中主要的显色物质,其在上、下表皮中的含量可能导致了橙色、橙红色与鲜红色的花色变化;而深红色与紫红色山丹的类胡萝卜素在有色体中的颜色被液泡中的花青素遮盖,这2种颜色山丹可能是类胡萝卜素与花青素共同显色。花青素生物合成途径基因的表达分析表明,LpDFR与LpbHLH2可能是引起山丹花色分化的关键基因。研究结果为山丹种质资源保护和创新性利用策略的制定提供了理论依据,为山丹遗传改良和新品种培育提供了潜在的种质资源。
An in-depth investigation in wild Lilium pumilum was conducted in Taigu District,Jinzhong City,Shanxi province,China.This study delineated the geographic distribution,the germplasm diversity and the flower color differentiation of L.pumilum.Preliminary investigations were conducted into the genetic underpinnings and molecular mechanisms that drive flower color variation among wild L.pumilum populations.These variations were evaluated from phenotypic,cytological,physiological and molecular perspectives.L.pumilum in Taigu was found distributing within a longitude range of 112°39′50″to 112°95′64″,a latitude range of 37°19′12″to 37°42′17″,and an altitude range of 963 to 1430 m.This species was not identified from Xiaobai town,while 683 plants were found in Yangyi town,394 in Fancun town and 246 in Houcheng town.Abundant phenotypic diversity is evident in plant height,stem color,flower color,hairy flower buds and seed color.The diversity in plant height is significantly correlated with altitude,whereas the diversity in flower and stem color was significantly correlated with geographical distribution of the plants.Using a color card and colorimeter,the five flower colors of L.pumilum were identified as orange,orange red,red,dark red and purple red.Orange red was the most abundant and accounts for 90.2%of the population.Xiaodian and Yangyi towns are the only locations where all five flower colors coexist.The color differences among these five flower colors are pronounced,with orange and orange red L.pumilum being the most vivid.Carotenoids in the plastids serve as the primary coloring substances found in L.pumilum.The varying carotenoid content in the upper and lower epidermis leads to changes in flower colors between orange,orange red and red varieties.In contrast,the dark red and purple red flowers are due to the carotenoids being largely obscured by anthocyanins present in the vacuoles.Expression analysis of genes involved in the anthocyanin biosynthesis pathway suggests that LpDFR and LpbHLH2 may be the key genes responsible for color differentiation.This study provides a theoretical foundation for the formulation of protection and innovative utilization strategies for L.pumilum germplasm resources and offers potential germplasm resources for genetic improvement and the cultivation of new varieties.
作者
刘鑫如
廖矿矿
郝渊鹏
韩美玲
贾建伟
杜方
LIU Xinru;LIAO Kuangkuang;Hao Yuanpeng;HAN Meiling;JIA Jianwei;DU Fang(College of Horticulture,Shanxi Agricultural University,Taigu 030031;College of Urban and Rural Construction,Shanxi Agricultural University,Taigu 030031;Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan 030006;Shanxi Hetianyue Agricultural Technology Service Company Limited,Lvliang 033299)
出处
《植物遗传资源学报》
北大核心
2025年第9期1858-1870,共13页
Journal of Plant Genetic Resources
基金
山西省基础研究计划资助项目(20210302123416)
山西农业大学横向科技项目(2023HX292)。
关键词
山丹
资源调查
色素
QRT-PCR
山西省
Lilum pumilum
resource investigation
pigment
qRT-PCR
Shanxi province
