摘要
目的 通过对不同产地19批柴胡药材研究,建立柴胡药材高效液相指纹图谱,提高其质量控制水平。方法 采用Sepax BR C184.6 mm×250 mm,以乙腈-水作为流动相进行梯度洗脱,ELSD检测器(T=105℃,F=3.0 L/min)。以3号峰柴胡皂苷a为参照,记录19批柴胡药材的HPLC图谱,将这些数据导入《中药色谱指纹图谱相似度评价软件系统2012版》(国家药典委员会),采用指纹图谱软件确定共有峰,建立19批柴胡药材的HPLC指纹图谱,标定9个共有峰,其中3号峰为柴胡皂苷a、6号峰为柴胡皂苷d,所有批次的柴胡药材在相似度上都达到了0.90以上。经验证与对照特征图谱具有较好的一致性。结果 该方法稳定可靠、重现性好,可用于柴胡药材质量控制方法,同时也为柴胡药材质量评价提供科学依据。结论 本文所建立的方法准确、可靠,可用于评价和控制不同产地柴胡药材的质量。
Objective To establish a fingerprint diagram of Chaihu through the study of 19 batches of Chaihu from different habitats and improve its quality control level.Methods Sepax BR C184.6 mmx250 mm column and acetonitrile water were used as the mobile phase for gradient elution,with an ELSD detector(T=105℃,F=3.0 L/min).Taking the No.3 chacharutin A as the reference to record the HPLC map of 19 batches of Chaihu and introduced it into the 2012 Edition of the Simplicate Evaluation Software System(National Pharmacopoeia Committee).The HPLC fingerprint map of 19 batches of Chaihu was established,and 9 co-peaks were calibrated.Among them,the Peak 3 was chaihu saponin a and the Peak 6 was Chaihu saponin d.Verification had good consistency with the control fingerprint map.Results This method is stable,reliable,good,which can be used for the quality control method of Chaihu and provides scientific basis for its quality evaluation.Conclusion The method established in this article is accurate and reliable,which can be used to evaluate and control the quality of Chaihu from different habitats.
作者
卫禹辰
朱丽玲
吴宁
何建华
孙晓熙
许汉林
WEI Yu-chen;ZHU Li-ling;WU Ning;HE Jian-hua;SUN Xiao-xi;XU Han-lin(College of Pharmacy,Hubei University of ChineseMedicine,Wuhan 430065;Wuhan YaoGu Technology Development Co.,LTD.,Wuhan 430079)
出处
《湖北中医药大学学报》
2025年第3期40-44,共5页
Journal of Hubei University of Chinese Medicine
基金
湖北中药质控标准物质研制及技术平台建立项目(项目编号:2020ACA007)。
