摘要
目的探究中枢神经系统特发性炎性脱髓鞘疾病(IIDDs)进程中,内皮素-1(ET-1)的表达水平与疾病残疾程度的相关性。方法纳入45例中枢神经系统脱髓鞘疾病患者,其中IIDDs患者25例,中枢神经系统血管性脱髓鞘疾病患者20例,同时纳入健康对照者10例。用ELISA的方法测定患者血清ET-1水平,并进一步分析IIDDs组患者血清ET-1水平与实验室检验指标、情绪认知功能及疾病残疾程度的相关性。结果(1)在三组中,IIDDs组血清ET-1含量最高(P<0.0001),血清ET-1在血管性脱髓鞘疾病组表达水平较健康对照组降低,但差异无统计学意义。(2)IIDDs中EDSS评分重度障碍患者血清ET-1水平较EDSS评分轻中度障碍患者升高(Z=-3.250,P=0.001);EDSS评分与血清ET-1水平(r_(s)=0.503,P=0.010)、脑脊液总蛋白(r_(s)=0.475,P=0.016)、脑脊液白蛋白(r_(s)=0.480,P=0.020)、脑脊液IgG(r_(s)=0.544,P=0.007)、脑脊液IgA(r_(s)=0.660,P=0.002)及脑脊液IgM(r_(s)=0.555,P=0.011)水平存在正相关性。(3)IIDDs组患者血清ET-1水平与脑脊液IgM水平(r_(s)=0.455,P=0.044)存在正相关性,与外周免疫指标无明显相关性(P>0.05)。结论血清ET-1水平反映了IIDDs临床症状的严重程度,且与外周免疫指标无明显相关性,但与疾病严重程度及脑脊液IgM水平存在正相关性,提示血清ET-1水平可反映中枢神经系统炎症程度,并在IIDDs发生发展中起到重要作用。
Objective To examine the relationship between the levels of endothelin-1(ET-1)and the severity of clinical symptoms in idiopathic inflammatory demyelinating diseases(IIDDs)of the central nervous system.Methods A total of 45 patients with central nervous system demyelinating diseases were enrolled in the study.Among them,25 patients were diagnosed with idiopathic IIDDs,20 had vascular demyelinating diseases of the central nervous system,and 10 healthy controls were also included.Serum ET-1 levels were assessed using ELISA,and a further analysis was conducted to investigate the correlation between these ET-1 levels,laboratory test results,and the degree of disease disability in the IIDDs patient group.Results①Of the three groups,ET-1 levels were highest in the IIDDs(P<0.0001).The expression level of ET-1 in the vascular demyelinating disease group was lower than in the healthy control group,but there was no statistical difference.②Serum ET-1 levels in patients with severe EDSS score group in IIDDs were higher than those in patients with mild to moderate EDSS score group(Z=-3.250,P=0.001).EDSS score was positively correlated with serum ET-1 levels(r_(s)=0.503,P=0.010),CSF total protein(r_(s)=0.475,P=0.016),CSF albumin(r_(s)=0.480,P=0.020),CSF IgG(r_(s)=0.544,P=0.007),IgA(r_(s)=0.660,P=0.002)and IgM(r_(s)=0.555,P=0.011)levels.③There was a positive correlation between serum ET-1 levels and CSF IgM levels(r_(s)=0.455,P=0.044)in IIDDs group.Serum ET-1 level showed no significant correlation with peripheral immune reaction(P>0.05).Conclusion Serum ET-1 levels reflect the severity of clinical symptoms in IIDDs and show no significant correlation with peripheral immune markers,however,exhibit a positive correlation with disease severity and cerebrospinal fluid IgM levels.These findings suggest that serum ET-1 levels may indicate the degree of central nervous system inflammation and play an important role in the development and progression of IIDDs.
作者
宋晨蕊
刘艳群
毕晓莹
Song Chenrui;Liu Yanqun;Bi Xiaoying(Dept of Neurology,The First Affiliated Hospital of Naval Medical University,Shanghai 200433;Dept of Neurology,The Third Hospital of Naval Medical University,Shanghai 201805)
出处
《安徽医科大学学报》
北大核心
2025年第7期1319-1324,共6页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:82371495)
海军军医大学第一附属医院青年培育项目(编号:2021JCQN10)。
关键词
中枢神经系统特发性炎性脱髓鞘疾病
内皮素-1
中枢神经系统血管性脱髓鞘
中枢神经系统炎症
残疾程度
情绪认知功能
idiopathic inflammatory demyelinating disease of the central nervous system
endothelin-1
vascular demyelinating diseases of the central nervous system
central nervous system inflammation
disability severity
emotional-cognitive function
