摘要
建立了超高效液相色谱-串联质谱(UPLC-MS/MS)同时测定食用植物油中27种光稳定剂的检测方法。食用植物油样品经甲醇-正己烷-丙酮(2∶1∶1,V/V/V)涡旋提取2 min,经QuEChERS分散固相萃取(dSPE)增强型去除脂质(EMR-Lipid)技术净化,过0.22μm聚四氟乙烯(PTFE)滤膜。目标物经ZORBAX Eclipse Plus C18色谱柱分离,甲醇-0.02%甲酸-5 mmol/L甲酸铵水溶液梯度洗脱,在电喷雾离子源(ESI)正离子模式下多反应监测模式(MRM)测定,外标法定量。结果表明,在线性范围内,27种光稳定剂的相关系数(r^(2))均大于0.9990,检出限(LODs)为4~200μg/kg,定量限(LOQs)为10~400μg/kg,平均加标回收率为82.0%~109.7%,相对标准偏差(RSDs)为0.6%~6.5%。该方法适用于食用植物油中27种光稳定剂的检测。
A method for the simultaneous determination of 27 light stabilizers in edible plant oil was developed using ultra high-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The edible plant oil samples were extracted by vortex extraction with methanol-(n-hexane)-acetone(2∶1∶1,V/V/V)for 2 min,purified by QuEChERS dispersive solid phase extraction(dSPE)enhanced matrix removal-lipid(EMR-Lipid)technology,and filtered through 0.22μm polytetrafluoroethylene(PTFE)membrane.The target compounds were separated by ZORBAX Eclipse Plus C18 column by gradient elution with mobile phase of methanol,0.02%formic acid,and 5 mmol ammonium formate aqueous solution,then determined by multi-reaction monitoring mode(MRM)under electrospray ionization(ESI)positive and quantified by external standard method.The results showed that the correlation coefficients(r^(2))of 27 light stabilizers were all over than 0.9990 in respective linear ranges,the limits of detection(LODs)were 4‒200μg/kg,the limits of quantification(LOQs)were 10‒400μg/kg,the average recoveries were 82.0%‒109.7%,and the relative standard deviations(RSDs)were 0.6%‒6.5%.The method was suitable for determining 27 light stabilizers in edible oil.
作者
邱月
陆嘉莉
李根容
陈锌
杨倩
QIU Yue;LU Jiali;LI Genrong;CHEN Xin;YANG Qian(Chongqing Academy of Metrology and Quality Inspection,Chongqing 401123,China)
出处
《分析试验室》
北大核心
2025年第7期1074-1082,共9页
Chinese Journal of Analysis Laboratory
基金
重庆市科技局科研院所绩效激励引导专项(cstc2022jxjl50001)
国家市场监督管理总局科技计划项目(2023MK106)
重庆市市场监督管理局科研计划项目(CQSJKJ2022040,CQSJKJDW2023003)资助。
