摘要
本研究通过藻(Chlorella sp.)—菌和菌(Talaromyces sp.、Aurantiochytrium sp.)—菌共培养对破囊壶菌(Thraustochytrid)中Aurantiochytrium sp.S3D(以下简称A.sp.S3D)生物量积累的影响。结果表明,光照显著促进破囊壶菌中A.sp.S3D的生长(P<0.01),但藻—菌共培养模式不利于破囊壶菌A.sp.S3D的生长。甘油—酵母提取物培养体系中,A.sp.S3D与T sp.混合培养生物量可达到(11.41±0.25)g/L,而葡萄糖—酵母提取物培养体系中,A.sp.S3D与A.sp.S3E以1∶4共培养时,生物量提升至(12.62±0.67)g/L。研究证实,酵母提取物是破囊壶菌培养的理想氮源,A.sp.S3D与A.sp.S3E培养更有利于破囊壶菌生物量的积累。
This study investigated co-culture strategies for enhancing biomass production in the oleaginous marine protist Aurantiochytrium sp.S3D,comparing algal-protist(Chlorella sp.)and protist-protist(Talaromyces sp.and A.sp.S3E)systems under varying light conditions and nutrient sources.While light significantly stimulated S3D growth(P<0.01),algal co-culture showed no synergistic effects.Optimal biomass yields were achieved in glycerol/YE medium with S3D-T.sp(11.41±0.25)g/L,and S3D-S3E co-culture at 1∶4 ratio in glucose/YE medium(12.62±0.67)g/L.These findings demonstrate that conspecific co-culture with organic nitrogen maximizes S3D productivity.
作者
刘倩
王润智
惠瑞雪
王秋珍
LIU Qian;WANG Runzhi;HUI Ruixue;WANG Qiuzhen(Ocean College,Hebei Agricultural University,Qinhuangdao 066003,China)
基金
河北省省属高校基本科研业务费研究项目(KY2023087)。
