摘要
【目的】筛选并鉴定一株具有抑制蓝靛果采后致腐真菌功能的乳酸菌,并研究其对蓝靛果采后保鲜效果的影响,为解决蓝靛果采后腐烂问题提供新的技术手段。【方法】从泡菜与发酵浆果中分离纯化乳酸菌后,筛选能抑制蓝靛果采后致腐真菌(链格孢霉与小孢根霉)的乳酸菌P-2,通过16S rRNA基因序列分析鉴定菌株。评估P-2的抗生素敏感性以及不同条件下无细胞上清液(cell-free supernatant,CFS)对致腐真菌抑制效果。最后以P-2的CFS作为保鲜剂对蓝靛果进行保鲜实验。【结果】在分离并纯化出的22株乳酸菌中,P-2菌株展现出最佳的抑菌效果,经鉴定为乳酸片球菌(Pediococcus acidilactici)。乳酸片球菌P-2对卡那霉素、庆大霉素与链霉素耐受,对其他抗生素敏感或中性敏感,其CFS在酸性条件下的抑菌活性较强,对蛋白酶敏感。P-2 CFS对链格孢霉和小孢根霉的MIC分别为12.8 mg/mL、25.6 mg/mL。与对照组相比,乳酸片球菌P-2的CFS可以降低蓝靛果的失重率和腐烂率,减缓蓝靛果在贮藏期间硬度、总酚、类黄酮以及维生素C含量的下降,同时可以使蓝靛果的货架期至少延长7 d。【结论】乳酸片球菌P-2的CFS具有抑制蓝靛果致腐真菌的作用,可作为一种绿色保鲜剂应用于浆果的采后保鲜中。
【Objective】To screen and identify a strain of lactic acid bacteria that can inhibit rot-causing fungi in post-harvest Lonicera caerulea L.,and to explore its effect on post-harvest preservation of L.caerulea L,this may provide new technical means to solve the problem of post-harvest rotting of L.caerulea L.【Method】After the isolation and purification of lactic acid bacteria from pickles and fermented berries,the P-2,which can inhibit the rot-causing fungi(Alternaria alternata and Rhizopus microsporus)in post-harvest L.caerulea L.,were screened and identified by 16S rRNA gene sequence analysis.To evaluate the antibiotic sensitivity of P-2 and the inhibitory effect of cell-free supernatant(CFS)on rot-causing bacteria under different conditions.At last,P-2 CFS was used as a preservative to keep L.caerulea L fresh.【Result】Among the 22 strains of isolated and purified lactic acid bacteria,P-2 strain showed the best bacteriostatic effect and was identified as Pediococcus acidilactici.P-2 is resistant to kanamycin,gentamicin and streptomycin,sensitive or neutral to other antibiotics,its CFS has strong antifungal activity under acidic conditions,and is sensitive to protease.The MIC of P-2 CFS against A.alternata and R.microsporus were 12.8 and 25.6 mg/mL,respectively.Compared with the control group,the CFS of P-2 reduced the weight loss rate and decay rate of L.caerulea L,slowed down the decline of hardness,total phenol,flavonoid and vitamin C content of L.caerulea L during storage,and prolonged the shelf life of L.caerulea L.for at least 7 d.【Conclusion】The CFS of P.acidilactici P-2 can inhibit the post-harvest rot-causing bacteria of L.caerulea L.,and can be used as a green preservative in the post-harvest preservation of berries.
作者
李德海
殷丽
周才雪
王泽童
孙常雁
LI De-hai;YIN Li;ZHOU Cai-xue;WANG Ze-tong;SUN Chang-yan(State Key Laboratory of Utilization of Woody Oil Resource,Northeast Forestry University,Harbin 150040;College of Life Sciences,North-east Forestry University,Harbin 150040;College of Materials Science and Chemical Engineering,Harbin University of Science and Technol-ogy,Harbin 150080)
出处
《生物技术通报》
北大核心
2025年第8期289-299,共11页
Biotechnology Bulletin
基金
黑龙江省自然科学基金面上项目(LH2022C007)
黑龙江省“百千万”工程科技重大专项(2020ZX07B01-3-1A)
中央高校基本科研业务费专项(2572019BA04)
哈尔滨市科技局创新研究基金(2017RAQXJ091)。
关键词
乳酸菌
蓝靛果
致腐真菌
抗真菌活性
链格孢霉菌
小孢根霉菌
筛选
保鲜
lactic acid bacteria
Lonicera caerulea L.
rot-causing fungi
antifungal activity
Alternaria alternata
Rhizopus microspora
screening
keep fresh
