摘要
目的探讨山柰酚促进炎症状态下人根尖乳头干细胞(stem cells from the apical papilla,SCAP)增殖和成骨的适宜浓度以及山柰酚对炎症状态下人SCAP成骨/成牙本质向分化的影响。方法采用酶消化法培养SCAP,流式细胞术检测细胞表面标志物。加入10 ng/mL肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α),建立炎症模型。设空白对照Control组、TNF-α组、TNF-α+山柰酚(0.001μM)组、TNF-α+山柰酚(0.01μM)组、TNF-α+山柰酚(0.1μM)组、TNF-α+山柰酚(1μM)组、TNF-α+山柰酚(10μM)组,进行CCK-8试剂盒克隆形成实验和碱性磷酸酶(Alkaline phosphatase,ALP)染色活性实验检测不同浓度山柰酚对SCAP增殖和ALP分泌水平的影响,得到山柰酚促进炎症状态下SCAP增殖和成骨的适宜影响浓度。茜素红染色检测适宜浓度下山柰酚对炎症状态下人根尖乳头干细胞成牙成骨的影响。结果CCK-8结果显示干预13天,0.001μM组、0.01μM组、0.1μM组能明显促进SCAP的增殖(P<0.05),而1μM组、10μM组对炎症状态下SCAP的增殖产生抑制作用(P<0.05)。与TNF-α组相比较,0.1μM、1μM组、10μM组可显著提高炎症状态下SCAP的ALP活性(P<0.05)。提示0.1μM是山柰酚促进炎症状态下SCAP增殖成骨的适宜影响浓度。茜素红染色表明0.1μM山奈酚可促进炎症状态下SCAP的矿化基质的合成能力。结论:0.1μM山柰酚可显著促进炎症状态下SCAP增殖成牙成骨的能力,该结果为山柰酚促进牙髓牙本质再生、牙根发育研究提供实验依据。
Objective To evaluate the optimal concentration of kaempferol for promoting the proliferation and osteogenesis of human stem cells from the apical papilla(SCAP)under inflammatory conditions,and to investigate its effects on osteogenic and dentinogenic differentiation potential.Methods SCAP were isolated and cultured using enzymatic digestion,with surface markers analyzed by flow cytometry.An inflammation model was induced using 10 ng/mL tumor necrosis factor-α(TNF-α).Groups included a blank control,TNF-α,and TNF-α combined with kaempferol at concentrations of 0.001,0.01,0.1,1,and 10μM.Cell proliferation and alkaline phosphatase(ALP)activity were assessed using CCK-8 assay kit colony formation experiments and ALP staining to investigate the effects of different concentrations of kaempferol on SCAP proliferation and ALP secretion levels,thereby determining the optimal concentration of kaempferol that promotes SCAP proliferation and osteogenesis under inflammatory conditions.Alizarin red staining was used to assess the effects of kaempferol at optimal concentrations on the odontogenesis and osteogenesis of SCAP under inflammatory conditions.Results CCK-8 results showed that after 13 days of treatment,kaempferol at 0.001,0.01,and 0.1μM significantly enhanced SCAP proliferation(P<0.05),whereas 1 and 10μM inhibited proliferation(P<0.05).ALP activity was notably increased in the 0.1,1,and 10μM groups compared with the TNF-α group(P<0.05),suggesting that 0.1μM is the optimal concen‐tration of kaempferol for promoting the osteogenic proliferation of SCAP under inflammatory conditions.Alizarin red staining demonstrated that 0.1μM kaempferol effectively promoted mineralized matrix synthesis in SCAP un‐der inflammation.Conclusion Kaempferol at 0.1μM significantly enhances the osteogenic and dentinogenic po‐tential of SCAP under inflammatory conditions,suggesting its potential application in regenerative endodontics and root development therapy.
作者
赵璐
沙比然·乃吉米丁
周晶
吴佩玲
Zhao Lu;Shabiran Najimiding;Zhou Jing;Wu Peiling(Department of Stomatology,The Second Affiliated Hospital of Xinjiang Medical University,Urumqi 830063)
出处
《口腔材料器械杂志》
2025年第3期147-152,共6页
Chinese Journal of Dental Materials and Devices
基金
新疆维吾尔自治区自然科学基金项目(2022D01C786)。
