摘要
早期胚胎发育关乎人口健康.异常胚胎发育过程可引发妊娠相关疾病、出生缺陷和发育源性疾病等.因此,解析人类早期胚胎发育过程和调控机理对于了解人类生命起源、探究病理性胚胎发生机制、提高相关疾病的诊疗效率至关重要.然而,由于伦理和技术限制,人类体内发育的正常胚胎难以直接用于科学研究,因此胚胎体外培养(in vitro culture,IVC)成为研究胚胎早期发育的重要技术.目前,IVC技术已被应用于小鼠、大鼠、兔、猪、猴和人等多种哺乳动物胚胎的发育过程研究.这项技术对发育生物学、遗传学、毒理学、再生医学等领域研究至关重要.本文对胚胎体外培养技术的发展进行回顾,总结不同IVC体系的关键技术以及影响因素,并探讨IVC技术面临的挑战和未来发展方向.
Early embryonic development is crucial for population health.Abnormal embryonic development can lead to pregnancy-related diseases,birth defects,and development-originated diseases.Therefore,deciphering the processes and regulatory mechanisms of early human embryonic development is crucial for understanding the origin of human life,exploring the mechanisms of pathological embryogenesis,and improving the diagnostic and therapeutic efficacy of related diseases.However,due to ethical and technical limitations,human embryos that are developed within the uterus are difficult to be directly used for scientific research.Therefore,embryo in vitro culture(IVC)has become an important technique for studying the early embryonic development.Currently,embryo IVC technologies have been applied to reveal the features of embryonic development in various mammals,including mice,rats,rabbits,pigs,monkeys,and humans.These technologies are vital to the fields of developmental biology,genetics,toxicology,and regenerative medicine.This article reviews the development of embryo IVC technologies,summarizes the methods and factors of different culture systems,and discusses the challenges and future directions.
作者
于坤元
刘子琛
翟晶磊
YU KunYuan;LIU ZiChen;ZHAI JingLei(State Key Laboratory of Stem Cell and Reproductive Biology,Key Laboratory of Organ Regeneration and Intelligent Manufacturing,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China;University of the Chinese Academy of Sciences,Beijing 100049,China;Beijing Institute of Stem Cell and Regenerative Medicine,Beijing 100101,China)
出处
《中国科学:生命科学》
北大核心
2025年第7期1319-1331,共13页
Scientia Sinica(Vitae)
基金
国家重点研发计划(批准号:2021YFA0805701)
国家自然科学基金优秀青年科学基金(批准号:82322026)资助。
关键词
早期胚胎发育
胚胎体外培养
原肠运动
早期器官发育
再生医学与器官制造
early embryonic development
in vitro culture of embryos
gastrulation
early organogenesis
regenerative medicine and organ manufacturing
