摘要
以蝴蝶兰花序轴腋芽为试材,在离体培养条件下,设置高温(昼夜温度30℃/28℃)和低温(昼夜温度24℃/18℃)环境,研究2个光照强度(200μmol·m^(-2)·s^(-1)和30μmol·m^(-2)·s^(-1))对蝴蝶兰‘大辣椒’花序轴腋芽生长发育、生理指标和内源激素的影响,以期探究蝴蝶兰花序轴腋芽快速诱导成苗的关键环境因子。结果表明:在低温强光条件下,花序轴腋芽进行生殖生长发育为完整花序,在高温强光、高温弱光和低温弱光条件下,均可以营养生长发育为幼苗。低温强光条件下,腋芽0.5 cm时,淀粉和可溶性糖明显高于其他处理,但淀粉含量却低于高温强光条件下腋芽的含量;玉米素(ZA)含量明显高于另外3个处理,且在1.0 cm和1.5 cm时明显降低;腋芽赤霉素(GA_(3))含量并没有明显高于其他3个处理,生长素(IAA)含量高于其他3个处理,并在后期的分化过程中持续增加,ZA/GA_(3)、ABA/GA_(3)的比值明显高于其他处理。在离体培养条件下,高温强光可快速启动腋芽并分化成苗,低温强光则促使其进行生殖生长再次分化为花序。
Taking the axillary buds of the flower spike of Phalaenopsis as experimental material,under in vitro culture conditions,two different temperature conditions(high-temperature,day/night temperature of 30℃/28℃;low-temperature,day/night temperature of 24℃/18℃)were set up.The effects of two light intensities(200μmol·m^(-2)·s^(-1)and 30μmol·m^(-2)·s^(-1))on the growth and development,physiological indices,and endogenous hormone levels of the axillary buds of flower stalk in Phalaenopsis‘Big Chili’were studied,in order to explore the key environmental factors for the rapid induction of seedling formation from the axillary buds of the flower spike in Phalaenopsis.The results showed that under low-temperature and high-light conditions,the axillary buds developed through reproductive growth into complete flower spikes.Conversely,under high-temperature/high-light,high-temperature/low-light,and low-temperature/low-light conditions,they underwent vegetative growth and developed into seedlings.In the low-temperature/high-light treatment,when the axillary buds reached 0.5 cm,the levels of starch and soluble sugars were significantly higher compared to other treatments,although starch content was lower than that observed under high-temperature/high-light conditions.The concentration of zeatin(ZA)was significantly higher than in the other three treatments,with a marked reduction observed at 1.0 cm and 1.5 cm in length.The level of gibberellic acid(GA_(3))in the axillary buds did not significantly differ from the other treatments,while indole-3-acetic acid(IAA)levels were higher than those in the other treatments,continuously increasing during the later stages of differentiation.The ratios of ZA/GA_(3)and abscisic acid(ABA)/GA_(3)were significantly greater than those in the other treatments.Under in vitro culture conditions,high-temperature and high-light could rapidly initiate the axillary buds and promote their differentiation into seedlings,while low-temperature and high-light conditions encourage reproductive growth,leading to further differentiation into flower spikes.
作者
许申平
李俊霖
梁芳
张燕
蒋素华
袁秀云
XU Shenping;LI Junling;LIANG Fang;ZHANG Yan;JIANG Suhua;YUAN Xiuyun(Institute of Bioengineering,Zhengzhou Normal University,Zhengzhou,Henan 450044)
出处
《北方园艺》
北大核心
2025年第15期108-116,共9页
Northern Horticulture
基金
河南省高等学校重点科研资助项目(24A220007)。
关键词
蝴蝶兰
离体培养
腋芽
植物激素
Phalaenopsis
in vitro culture
axillary bud
phytohormone
