摘要
组织培养是开展花生(Arachis hypogaea L.)遗传转化的重要环节,但花生胚性愈伤组织的分化受到基因型限制。在花生遗传转化体系的建立过程中,从9份种质资源中筛选出愈伤分化能力强的品种漯花22号,并对漯花22号分化过程中的T1型愈伤(易分化成苗的胚性愈伤)、T2型愈伤(难分化成苗的非胚性愈伤)进行转录组测序分析。与对照(分化0 d的愈伤)相比,T1、T2型愈伤中分别具有1792个和868个差异表达基因(DEGs,differentially expressed genes)。GO条目富集分析表明,T1型愈伤的DEGs主要富集在分生组织维持和干细胞群维持等条目;T2型愈伤的DEGs主要富集在苯丙烷生物合成和代谢途径等条目。蛋白质家族分析显示,T1、T2型愈伤的DEGs包含2459个蛋白质编码基因,其中细胞色素P450家族显著富集。通过蛋白质-蛋白质互作(PPI,protein-protein interaction)网络分析,挖掘到5个枢纽基因AhAE3ZZG、AhP17M1H、AhA6R79F、AhZFZ3ZQ和AhHMN99B,这些基因可能在促进花生胚性愈伤组织分化过程中起重要作用。本研究结果将为进一步探究影响花生胚性愈伤组织分化过程中的关键基因、解析花生愈伤分化形成再生植株的分子机理提供科学依据。
Tissue culture is a crucial step in the genetic transformation of peanut(Arachis hypogaea L.).However,the differentiation of peanut callus is restricted by genotype.In this study,Luohua 22,a cultivar with strong callus differentiation ability,was screened from 9 germplasm resources through establishing a peanut genetic transformation system.Transcription sequencing analysis were carried out using T1 callus(embryogenic callus prone to differentiate into seedlings),T2 callus(non-embryogenic callus difficult to differentiate into seedlings)during the differentiation process of Luohua 22,and callus at 0 day of differentiation was set as control(CK).Compared with CK,there were 1792 and 868 differentially expressed genes(DEGs)in T1 and T2 types of callus,respectively.The GO term enrichment analysis showed that the DEGs in T1 type of callus were mainly enriched in the terms of meristematic organization and stem cell population maintenance,while those in T2 type of callus were mainly enriched in the terms of phenylpropane biosynthesis and metabolic term.Protein family analysis showed that DEGs in T1 and T2 types of callus contained 2459 protein-coding genes,among which the cytochrome P450 family was significantly enriched.By protein-protein interaction(PPI)network analysis,five key genes AhAE3ZZG,AhP17M1H,AhA6R79F,AhZFZ3ZQ and AhHMN99B,were mined,which might play important roles in promoting peanut callus differentiation.The results of this study would provide a scientific basis for furhter exploring the key genes affecting the differentiation process of peanut cotyledon node callus,and the subsequent analysis of the molecular mechanism of peanut callus differentiation to form regenerated plants.
作者
吴日连
上官小霞
吴翠翠
夏芝
张晓玲
李换丽
兰刚
潘转霞
侯保国
吕贝贝
李朋波
WU Rilian;SHANGGUAN Xiaoxia;WU Cuicui;XIA Zhi;ZHANG Xiaoling;LI Huanli;LAN Gang;PAN Zhuanxia;HOU Baoguo;LYU Beibei;LI Pengbo(Institute of Cotton Research,Shanxi Agricultural University,Yuncheng 044000;College of Agriculture,Shanxi Agricultural University,Jinzhong 030800)
出处
《植物遗传资源学报》
北大核心
2025年第9期1809-1823,共15页
Journal of Plant Genetic Resources
基金
山西省科技重大专项揭榜挂帅项目(202201140601025-4-03)
山西农业大学科技创新提升工程项目(CXGC2023054)
山西农业大学棉花研究所博士基金项目(SBSJJ2023-02)。
