摘要
以西瓜耐盐材料“PI296341”和盐敏感材料“ZYL”为试材,采用转录组测序的方法,研究了0、300 mmol·L^(-1) NaCl处理下幼苗叶片基因表达水平的变化,以期探讨西瓜耐盐分子机制并挖掘耐盐相关基因。结果表明:在4个处理中鉴定出差异表达基因73个,其中有29个基因在盐敏感材料中对盐胁迫不敏感,而在耐盐材料中对盐胁迫表现出显著的响应。如编码蓝铜蛋白的Cl97C09G165080、编码BHLH转录因子的Cla97C03G059090、编码乙烯应答转录因子的Cla97C06G116550、编码水通道蛋白的Cla97C04G075690、编码NAC转录因子的Cla97C05G106120、编码F-box转录因子的Cla97C10G200700、编码WRKY转录因子的Cla97C01G021390、Cla97C07G141890、Cla97C10G205870和编码谷胱甘肽转移酶的Cla97C05G083070和Cla97C06G123950,这些基因均上调表达,推测这些基因可能在西瓜响应盐胁迫过程中发挥正向调节功能。
Taking the salt-tolerant material‘PI296341’and the salt-sensitive material‘ZYL’of watermelon as the test materials,the changes in gene expression levels of seedling leaves treated with 0 and 300 mmol·L^(-1) NaCl were studied by transcriptome sequencing,with the aim of exploring the molecular mechanism of salt tolerance in watermelon and unearth salt-tolerant related genes.The results showed that 73 differentially expressed genes were identified in the four experimental groups.Among them,29 genes were insensitive to salt stress in salt-sensitive materials but showed significant responses to salt stress in salt-tolerant materials.Such as Cl97C09G165080 encoding ceruloplasmin,Cla97C03G059090 encoding BHLH transcription factor,Cla97C06G116550 encoding ethylene response transcription factor,Cla97C04G075690 encoding aquaporin,and Cla97C05G encoding NAC transcription factor 106120,Cla97C10G200700 encoding F-box transcription factor,Cla97C01G021390,Cla97C07G141890,Cla97C10G205870 encoding WRKY transcription factor,and Cla97C05G083070 encoding glutathione transferase Cla97C06G123950,all these genes are up-regulated.It was speculated that these genes may play a positive regulatory role in the process of watermelon responding to salt stress.
作者
贾云鹤
JIA Yunhe(Horticulture Branch,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150069)
出处
《北方园艺》
北大核心
2025年第15期1-11,共11页
Northern Horticulture
基金
黑龙江省属科研业务费资助项目(CZKYF2025-1-B016)
黑龙江省重点研发计划资助项目(SC2022ZX02C0202)
国家西甜瓜产业技术体系哈尔滨综合试验站资助项目(CARS-25)
黑龙江省西甜瓜现代农业产业技术协同创新推广体系资助项目
黑龙江省农业科技创新跨越工程资助项目(CX23GG05)。
关键词
西瓜
盐胁迫
转录组
分子机制
watermelon
salinity stress
transcriptome
molecular mechanism
