摘要
为明确凉粉草不同生长期的成分积累和分配规律,以闽粤地区的主栽品种闽选1号为试验材料,在其营养生长阶段的6个不同时期进行采收,对比叶和茎的生物量、多糖、总黄酮和总多酚等成分含量及分配比例,分析各指标变化规律和相关性。结果表明,随生长期推进,凉粉草生物量主要向茎部积累。在营养生长后期(S6时期),茎的多糖、总黄酮和总多酚累积量均达最大值,分别为27.90、36.89和3.96 g/株,分配比例分别达到叶的3.85、4.75和8.02倍;叶和茎的1,1-二苯基-2-三硝基苯肼自由基(DPPH·)清除率分别为88.91%和91.03%,2,2′-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐自由基(ABTS·+)清除率分别为76.09%和88.45%,以茎的体外抗氧化活性较高。因此,茎是凉粉草成分的主要积累部位,S6时期是保障凉粉草品质的最佳采收期。相关性分析表明,茎的多糖含量与株高、茎鲜重呈显著相关,茎的多糖和总黄酮含量均与茎干重呈极显著正相关;叶和茎的ABTS·+清除率均与相应部位的总黄酮含量呈显著正相关,茎的DPPH·清除率与茎中多糖和总黄酮含量呈显著正相关、与总多酚含量呈极显著正相关。说明茎部多糖、总黄酮及总多酚含量可作为表征体外抗氧化活性和凉粉草品质的指标。因此,促进茎的生长和多糖、类黄酮等成分的积累,是后期凉粉草栽培、管理等技术的研发重点。本研究为凉粉草采收期管理提供了理论依据。
To clarify the accumulation and distribution of components at different growth stages of Mesona chinensis Benth,the main cultivated variety Minxuan 1(MX1)in Fujian and Guangdong Province was used as the experimental material.The seedlings were harvested at six different nutritional growth stages(S1 to S6).The biomass of plant leaves and stems and the contents of components such as polysaccharides,total flavonoids,and total polyphenols were compared.We also analyzed the changes of indices with time,and the correlations among various indicators.The results showed that the biomass weight of stems was the higher than that of leaves.At the S6 stage,the accumulation quantities of polysaccharides,total flavonoids,and total polyphenols in the stems were 3.85,4.75,and 8.02 times of that in the leaves,respectively(reaching up to 27.90,36.89,and 3.96 g·plant−1,respectively).The scavenging rates of DPPH·by substances extracted from leaves and stems were 88.91%and 91.03%,respectively,while the scavenging rates of ABTS·+were 76.09%and 88.45%,respectively.The above results indicated a high antioxidant activity in vitro for the substances extracted from stems.Thus,the stems was the main part of MX1 upon its medical utilization,and the S6 stage was the optimal harvesting period to ensure the quality of MX1.The concentrations of polysaccharides in stems were significantly positively correlated with plant height,fresh weights of stems,while the polysaccharide and total flavonoid content of stems were extremely significantly positively correlated with the dry weight of stems.The scavenging rate of ABTS·+both in leaves and stems were significantly positively correlated with the individual flavonoid concentration.In stems,the scavenging rate of DPPH·was significantly positively correlated with the concentrations of polysaccharides and total flavonoids,while extremely significantly positively correlated with total polyphenols.The above results indicated that the concentrations of polysaccharides,total flavonoids,and total polyphenols in stems were main indices for the antioxidant activity and quality of MX1.In conclusion,the cultivation technologies to increase stem biomass and concentrations of components such as polysaccharides and flavonoids in stems of MX1 are main concerns in future.The results of this study provide a theoretical basis for the management of harvesting period for MX1.
作者
朱雁鸣
林文明
林凤芳
牛雨晴
郑梅霞
陈宏
朱育菁
苏海兰
ZHU Yanming;LIN Wenming;LIN Fengfang;NIU Yuqing;ZHENG Meixia;CHEN Hong;ZHU Yujing;SU Hailan(Institute of Crop Sciences(Fujian Germplasm Resources Center),Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013;Wuping County Economic Crop Technology Promotion Station,Longyan,Fujian 364300)
出处
《核农学报》
北大核心
2025年第9期2040-2048,共9页
Journal of Nuclear Agricultural Sciences
基金
国家重点研发计划(2022YFD1600302)
福建省科技计划重大专项(2022NZ029017)。
关键词
凉粉草
多糖
总黄酮
总多酚
生长期
Mesona chinensis
polysaccharides
total flavonoids
total polyphenols
different growth periods
