摘要
鉴于由荞麦引发的食物过敏问题日益突出,为精准识别和定量食品中的荞麦过敏原,本研究采用液相串联高分辨质谱技术,对荞麦中主要致敏蛋白(2S白蛋白和13S球蛋白)酶解后的样品进行数据采集与分析;通过考察肽段的特异性、灵敏度和精密度等,筛选出VQVVGDEGK和AIPVEVLANSYDISTK等5条荞麦专属肽段;采用液相串联三重四极杆质谱(LC-MS/MS)构建食品中荞麦过敏原的准确定量分析方法,并开展系统的方法学有效性评估。结果表明,该方法具有良好的特异性、灵敏度[定量限(LOQ)为5 mg·kg^(-1)]、准确度(60.3%~126.4%)和精密度(日内和日间变异系数<19.8%)。此外,应用该方法检测分析面条、粥、饮料和饼干等样品中的荞麦过敏原含量,发现部分样品中存在荞麦过敏原的隐形污染风险。本研究为荞麦过敏原的检测与风险防控提供了方法与技术支持。
The issue of buckwheat-induced food allergies has become increasingly prominent.To accurately identify and quantify buckwheat allergens in food,this study employed liquid chromatography coupled with high-resolution mass spectrometry to collect and analyze data from enzymatic digests of the major allergenic proteins in buckwheat(2S albumins and 13S globulins).The results showed that five buckwheat-specific peptides,such as VQVVGDEGK and AIPVEVLANSYDISTK were selected,by evaluating the specificity,sensitivity,and precision of the peptide fragments.Liquid chromatography coupled with tandem triple quadrupole mass spectrometry(LC-MS/MS)was used to establish an accurate quantitative analysis method for buckwheat allergens in food,and a systematic evaluation of the method’s effectiveness was conducted.The results demonstrated that the method exhibits excellent specificity,sensitivity(quantification limit of 5 mg·kg^(-1)),accuracy(60.3%-126.4%),and precision(intra-day and inter-day variation coefficients<19.8%).Furthermore,the method was successfully applied to the detection and analysis of buckwheat allergen content in samples such as noodles,porridges,beverages,and cookies,which revealed the potential risk of hidden buckwheat allergen contamination in some samples.This study provides methodological and technical support for the detection and risk control of buckwheat allergens.
作者
孟俊宏
杨沛洁
赵晗
林浩鹏
关文强
王凤玲
杨术鹏
李熠
MENG Junhong;YANG Peijie;ZHAO Han;LIN Haopeng;GUAN Wenqiang;WANG Fengling;YANG Shupeng;LI Yi(School of Biotechnology and Food Science,Tianjin University of Commerce/Tianjin Key Laboratory of Food Biotechnology,Tianjin 300000;Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193)
出处
《核农学报》
北大核心
2025年第9期1986-1994,共9页
Journal of Nuclear Agricultural Sciences
基金
中国农业科学院青年创新专项(Y2023QC13)
中国农业科学院农产品加工研究所青年创新专项(CAAS-ASTIP-Q2022-IFST-01)。
关键词
荞麦
食物过敏
液相串联质谱
定量分析
致敏蛋白
buckwheat
food allergy
liquid chromatography-mass spectrometry
quantitative analysis
allergenic protein
