摘要
通过建立双抗体夹心ELISA法测定食物中花生过敏原蛋白成分,为进出口食品花生过敏原成分检测和由花生导致的食物过敏性疾病的预防提供技术基础。提取花生总蛋白,免疫小鼠制备抗花生总蛋白的多克隆抗体,用该抗体包被酶标板,并用生物素标记该多抗,从而建立双多抗体夹心ELISA法;自制花生总蛋白标准品并检测该方法的灵敏度,同时检测15种食品中是否含有花生蛋白成分。成功地研制出双抗体夹心ELISA法检测食品中花生过敏原蛋白成分,具有特异性,其最低检出限为8ng/mL,标准曲线在8ng/mL～125ng/mL范围内线性良好;13种食品检测结果与食物过敏原标签标注内容相符,而2种标示不详的食品检测结果均呈现阳性。
To develop a new method with sandwich-antibody enzyme linked immunosorbent assay （ELISA） to detect peanut allergen protein trace in food products,which will provide a technical tool to prevent peanut allergy. The total peanut protein was extracted and further analyzed by SDS-PAGE. The Balb/c mice were immuned with the extracts of peanut proteins and the highly-titrated polyclonal IgG antibodies against them were isolated and coupled with biotin for sandwich ELISA kit. Fifteen food products were tested using this method. Our results indicated that the standard curve is linear with peanut allergen protein concentration from 8 ng/mL-125 ng/mL and the detection limit is 8 ng/mL of peanut allergen protein. We found that 13 food were detected with the peanut allergen protein, which were consistent with the food allergen labels from their manufactures. However, two foods without food allergen label were detected to contain peanut allergen protein.
出处
《食品研究与开发》
CAS
北大核心
2009年第6期110-114,共5页
Food Research and Development
基金
深圳非共识项目(2007)
深港创新圈计划项目(2007)
