摘要
根状茎表型及显微结构是根状茎类中药材鉴定的重要依据。本研究对29份不同产地的药用黄精种质资源开展根状茎表型性状及显微特征观察。结果表明,不同产地黄精表型性状特征存在较大差异,其中鲜重的变异系数最大(77.95%),色度变异系数最小(8.78%)。在遗传多样性分析中,芽头直径的遗传多样性指数最高(2.0646)。主成分分析与产量相关性状指标具有较高的载荷率(分别为0.879、0.727、0.857、0.838、0.732)。根状茎表型聚类分析结果将黄精种质资源分为3个类别。此外,所选品种的显微结构维管束和黏液细胞相关特征也均存在差异。综上所述,不同产地黄精在表型性状特征和显微结构特征上均存在较大差异,为黄精种质资源的筛选与评价、品种鉴别提供理论依据。
Phenotypic traits and microscopic structures of rhizomes as key indicators for the identification of medici⁃nal rhizome herbs.This study conducted observations on the phenotypic traits and microscopic characteristics of rhizomes of Polygonatum spp.germplasm resources from 29 different origins.The results indicated that there were significant variations in phenotypic traits among Polygonatum spp.from different regions.Among them,the coefficient of variation of fresh weight was the highest(77.95%),and that of chromaticity was the lowest(8.78%).In the genetic diversity analysis,the bud diameter exhibited the highest genetic diversity index(2.0646).Principal component analysis revealed high loading rates for yield-related trait indicators(0.879,0.727,0.857,0.838,and 0.732,respectively).Cluster analysis of rhizome phenotypic traits categorized the Polygonatum spp.germplasm resources into three distinct groups.Additionally,variations were observed in the microscopic structures,particularly in the vascular bundles and mucilage cells of the selected varieties.In conclusion,significant differences exist in both phenotypic traits and microscopic struc⁃tures of Polygonatum spp.from different regions,providing a theoretical foundation for the screening and evaluation of the germplasm resources,as well as for the variety identification.
作者
袁帅
杨赛
成宏
张静
周昌龙
王洲羿
李佳
刁英
胡中立
Yuan Shuai;Yang Sai;Cheng Hong;Zhang Jing;Zhou Changlong;Wang Zhouyi;Li Jia;Diao Ying;Hu Zhongli(School of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430023,Hubei,China;Hubei Enshi Selenium-rich Resource Observation and Research Station,Enshi 445023,Hubei,China;Nursing Department of Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei,China;Enshi Tujia and Miao Autonomous Prefecture Central Hospital,Enshi 445099,Hubei,China;The First Affiliated Hospital of Chongqing Medical University,Chongqing 402160,China;Huanggang Hospital of TCM,Huanggang 438000,Hubei,China;College of Life Sciences,Wuhan University,Wuhan 430072,Hubei,China)
出处
《生物资源》
2025年第3期287-295,共9页
Biotic Resources
基金
武汉轻工大学人才引进科研启动基金(2021RZ099)。
关键词
黄精
种质资源
根状茎
表型性状
显微结构
Polygonatum spp.
germplasm resources
rhizome
phenotypic trait
microscopic structure