摘要
【目的】探究丝裂原活化蛋白激酶激酶6(mitogen-activated protein kinase kinase6,MAP2K6)基因在湖羊不同发育阶段背最长肌组织中的表达水平,分析该基因的多态性与湖羊生长性状之间的相关性,以期为湖羊的生长性状分子育种提供新的标记资源。【方法】利用实时荧光定量PCR检测MAP2K6基因在湖羊(n=15)不同发育阶段背最长肌组织中的表达情况;通过Illumina OvineSNP 50K BeadChip检测湖羊(n=3024)MAP2K6基因的单核苷酸多态性(SNP),利用一般线性模型分析MAP2K6基因SNP位点与湖羊(n=1974)生长性状间的关联性,并使用R语言corrplot包计算湖羊体重与各体尺指标的相关系数。【结果】实时荧光定量PCR检测结果显示,湖羊背最长肌组织中MAP2K6基因表达量在初生到4月龄阶段逐渐升高,且3、4月龄的表达量均极显著高于初生、45日龄和6月龄(P<0.01)。湖羊MAP2K6基因中共检测到2个位点:rs414959578G>A和rs426057803A>G。关联分析结果显示,MAP2K6基因rs414959578G>A位点对湖羊5月龄体重、体高、体斜长、胸围、胸深、胸宽、十字部高、腰角宽,以及6月龄胸围、背膘厚有显著或极显著影响(P<0.05;P<0.01);rs426057803A>G位点对湖羊3月龄管围,5月龄胸围、管围和十字部高以及6月龄背膘厚有显著或极显著影响(P<0.05;P<0.01)。相关性分析结果显示,湖羊体重与体尺指标间存在显著正相关(P<0.05),但6月龄湖羊体斜长与6月龄胸宽、腰角宽,5月龄管围与6月龄腰角宽均不存在显著相关(P>0.05)。【结论】MAP2K6基因与湖羊背最长肌的发育相关,rs414959578G>A和rs426057803A>G位点对湖羊生长性状有显著影响。研究结果可为湖羊生长性状分子标记的挖掘和利用提供一定的理论依据。
【Objective】The aim of this study was to investigate the expression of mitogen-activated protein kinase kinase 6(MAP2K6)gene in longissimus dorsi muscle of Hu sheep at different developmental stages,and analyze the correlation between the polymorphisms of MAP2K6 gene and the growth traits of Hu sheep,so as to provide novel marker resources for the molecular breeding of growth traits in Hu sheep.【Method】Real-time quantitative PCR was utilized to analyze the expression of MAP2K6 gene in longissimus dorsi muscle of Hu sheep(n=15)at various developmental stages.The Illumina OvineSNP 50K BeadChip was used to detect the single nucleotide polymorphisms of MAP2K6 gene in Hu sheep(n=3024).A general linear model was used to analyze the relationship between MAP2K6 gene SNP and growth traits in Hu sheep(n=1974).The correlation coefficient between body weight and body size of Hu sheep was calculated by R language corrplot package.【Result】The results of Real-time quantitative PCR showed that the expression of MAP2K6 gene in longissimus dorsi muscle of Hu sheep gradually increased from birth to 4-month-old.The expression of MAP2K6 gene at 3 and 4-month-old was extremely significantly higher than that at birth,45-day-old,and 6-month-old(P<0.01).Two SNPs(rs414959578 G>A and rs426057803 A>G)of MAP2K6 gene in Hu sheep were detected.The association analysis results indicated that rs414959578 G>A of MAP2K6 gene had significant or extremely significant effects on the body weight,body height,body length,chest circumference,chest depth,chest width,cross height,and waist angle width at 5-month-old,as well as chest circumference and backfat thickness at 6-month-old in Hu sheep(P<0.05 or P<0.01).rs426057803 A>G of MAP2K6 gene had significant or extremely significant effects on the cannon circumference at 3-month-old,and chest circumference,shin circumference,and cross height at 5-month-old,as well as the backfat thickness at 6-month-old in Hu sheep(P<0.05 or P<0.01).Correlation analysis results revealed that there was significant positive correlations between body weight and body size indicators in Hu sheep(P<0.05).However,there was no significant correlation between body length and chest width/waist angle width at 6-month-old,or shin circumference at 5-month-old and waist angle width at 6-month-old(P>0.05).【Conclusion】MAP2K6 gene was associated with the development of longissimus dorsi muscle in Hu sheep,and rs414959578 G>A and rs426057803 A>G had significant influence on the growth traits in Hu sheep.The results could provide a theoretical basis for identifying and utilizing molecular markers for growth traits in Hu sheep.
作者
朱硕
孙敬华
鲍晶晶
尚明玉
刘天义
熊金珂
杨培福
姚书海
孙歆恬
贺建宁
杨会国
张莉
ZHU Shuo;SUN Jinghua;BAO Jingjing;SHANG Mingyu;LIU Tianyi;XIONG Jinke;YANG Peifu;YAO Shuhai;SUN Xintian;HE Jianning;YANG Huiguo;ZHANG Li(College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China;State Key Laboratory of Animal Biotech Breeding,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Livestock Research Institute,Academy of Animal Science of Xinjiang Uygur Autonomous Region,Urumqi 830011,China)
出处
《中国畜牧兽医》
北大核心
2025年第8期3695-3706,共12页
China Animal Husbandry & Veterinary Medicine
基金
内蒙古自治区揭榜挂帅(2022JBGS0024)
中国农业科学院科技创新工程(ASTIP-IAS01)
新疆维吾尔自治区天池英才计划人才项目(2023-28)。
