摘要
采用响应面法优化超速破碎提取万寿菊中叶黄素酯的提取工艺参数,并研究该提取方法提取的叶黄素酯提取物抗癌生物活性。在单因素实验基础上,确定超速破碎提取次数、提取时间、料液比3个因素为主要影响提取工艺的因素,以叶黄素酯提取率为评价指标,研究超速破碎提取的最佳工艺参数,同时设置不同浓度叶黄素酯(20、40、80μg/mL),通过细胞存活率和凋亡率检测实验研究叶黄素酯对HeLa细胞生长的影响。当超速破碎提取法提取转速为3500 r/min,提取次数为3次,提取时间为9 min,料液比为1∶19(g/mL),得到叶黄素酯提取率为15.66%。通过CCK-8试剂盒检测细胞存活率,FITC-Annexin V/PI流式细胞仪检测细胞凋亡率,实验结果表明,叶黄素酯对HeLa细胞生长具有抑制作用,随着叶黄素酯浓度增加,其抑制效果增强,其中当叶黄素酯浓度为80μg/mL时,HeLa细胞存活率降低近50%。超速破碎提取法能效高、低耗提取万寿菊中叶黄素酯,为工业化生产提供技术支撑,同时研究结果表明万寿菊提取物中叶黄素酯能够有效抑制Hela细胞生长。
This study aims to optimize the parameters for ultra-speed crushing-assisted extraction of lutein esters from marigold flowers using response surface methodology,and to evaluate the anticancer bioactivity of the resulting extract.Based on single-factor experiments,three critical parameters:number of extraction cycles,extraction time,and solid-to-liquid ratio,were selected for process optimization,with lutein esters yield as the evaluation metric,Additionally,lutein esters solutions at concentrations of 20,40,and 80μg/mL were tested to assess their effects on HeLa cell viability by CCK-8 assay and apoptosis by FITC-Annexin V/PI flow cytometry.The optimal extraction conditions were determined as follows:rotational speed of 3500 r/min,3 extraction cycles,9 min of extraction time,and a solid-to-liquid ratio of 1∶19(g/mL),achieving a lutein esters extraction yield of 15.66%.Cell viability was measured by CCK-8 kit,and the apoptosis rate was detected by FITC-Annexin V/PI flow cytometry.Experimental results demonstrated dose-dependent inhibitory effects of lutein esters on HeLa cell proliferation;with the increase of lutein esters concentration,the inhibitory effect was enhanced.At 80μg/mL,lutein esters reduced HeLa cell viability by approximately 50%.The ultra-speed crushing-assisted extraction method enables efficient and low-cost extraction of lutein esters from marigold flowers,providing a viable strategy for industrial-scale production.Furthermore,the lutein esters extract exhibits potent inhibitory activity against HeLa cell growth,highlighting its potential as a natural anticancer agent.
作者
陈本书
罗爱芹
丛勇
梁阿新
李紫媛
罗茂国
孙立权
CHEN Ben-shu;LUO Ai-qin;CONG Yong;LIANG A-xin;LI Zi-yuan;LUO Mao-guo;SUN Li-quan(School of Life Science;Biological&Medical Engineering Core Facilities,Beijing Institute of Technology,Beijing 100081,China)
出处
《化学试剂》
2025年第8期52-58,共7页
Chemical Reagents
关键词
万寿菊
叶黄素酯
超速破碎提取
响应面法
抗癌
marigold
lutein esters
ultra-speed crushing extraction
response surface methodology
anti-cancer
