摘要
为实现吴兴铁线莲产业化生产,加快推广应用进程,建立吴兴铁线莲植物组织培养再生体系是最佳途径,以两年生吴兴铁线莲带芽茎段为外植体,进行组培快繁的研究。结果表明,灭菌处理以70%酒精灭菌10 s和10%次氯酸钠灭菌10 min混合处理效果最佳,出芽率达到70.67%,污染率为17.33%;启动培养以1/2MS培养基较适合,芽苗生长情况良好,不仅健壮且未出现黄化和褐化现象;增殖培养效果以MS+2 mg·L^(-1)6-BA+0.3 mg·L^(-1)IBA+2 g·L^(-1)水解酪蛋白最佳,增殖系数达到4.73;生根培养以1/2MS+0.5 mg·L^(-1)IBA+0.01 mg·L^(-1)NAA最佳,生根率达到79.67%。本研究建立了吴兴铁线莲完整的组培技术体系,打破了其地域限制,为其推广应用及商业化开发提供了理论基础,也为其后期的跨学科合作提供了一定支撑。
In order to realize industrial production and accelerate the popularization and application process,it is the best way to establish the plant tissue culture system of Clematis huchouensis.In this study,two-year-old stem segments of clematis from Xing Wu with buds were used as explants for tissue culture and rapid propagation.The results showed that the mixed treatment of 70%alcohol sterilization for 10 s and 10%sodium hypochlorite sterilization for 10 min was the best,with a germination rate of 70.67%and a pollution rate of 17.33%.1/2MS medium is more suitable for start-up culture,and the sprouts grow well,not only robust,but also without yellowing and browning.In the multiplication culture,MS+2 mg·L^(-1)6-BA+0.3 mg·L^(-1) IBA+2 g·L^(-1) hydrolyzed casein was the best,and the multiplication coefficient was 4.73.The best rooting culture medium was 1/2 ms+0.5 mg·L^(-1) IBA+0.01 mg·L^(-1) NAA,and the rooting rate was 79.67%.This study established a complete tissue culture technology system for Clematis huchouensis,breaking its geographical limitations.It provides a theoretical basis for its promotion and commercial development,and also offers certain support for its interdisciplinary cooperation in the later stage.
作者
石坚
邱国强
吴燕芬
汪惠峰
李建
张峰
SHI Jian;QIU Guoqiang;WU Yanfen;WANG Huifeng;LI Jian;ZHANG Feng(Deqing County Ecological Forsetry Comprehensive Center,Huzhou,Zhejiang 313200,China;Hangzhou Hongsen Forestry Survey Planning and Design Company Limited,Hangzhou,Zhejiang 310000,China;Zhejiang Forest Resources Monitoring,Hangzhou,Zhejiang 310000,China)
出处
《天津农业科学》
2025年第6期27-32,共6页
Tianjin Agricultural Sciences
基金
德清县野生植物资源调查服务采购项目(ZJXRDQ-2023-JC62)。
关键词
吴兴铁线莲
灭菌
组培快繁
培养基
Clematis huchouensis Tamura
sterilization
tissue culture
culture medium
