摘要
以L-天冬氨酸为单体通过缩聚反应合成了高分子量的聚琥珀酰亚胺。采用γ氨基丁酸和乙醇胺对其胺解,然后N-羟基琥珀酰亚胺(NHS)改性,得到羧基活化同时羟化的水溶性聚天冬氨酸。同时,以超支化聚乙烯亚胺(PEI)为稳定剂,在硼氢化钠的作用下利用化学还原法制备了含有银纳米簇的PEI-AgNCs溶液。两种材料在水溶液中混合,30 s内即可形成酰胺键交联的水凝胶。采用扫描电镜、电感耦合等离子体技术、MTT比色法和涂布平板法对水凝胶的形貌、银离子缓释、生物相容性和抗菌性能进行了表征。实验结果表明,聚天冬氨酸与PEI-AgNCs混合形成的水凝胶,比单独与PEI形成的水凝胶生物相容性更好,且对大肠杆菌和金黄色葡萄球菌的抗菌效果也更强。
High molecular weight polysuccinimide was synthesized by polycondensation reaction of L-aspartic acid.Followed by ammonolysis by gamma aminobutyric acid and ethanolamine and reaction with N-hydroxysuccinimide(NHS),the water soluble polyaspartic acid materials with hydroxyl and activated-COOH were obtained.Meanwhile,hyperbranched polyethyleneimine(PEI)-stabilized silver nanoclusters(PEI-AgNCs)were fabricated in the presence of sodium borohydride by chemical reduction method.When these two materials are mixed in aqueous solution,the amide crosslinked hydrogels can be formed within 30 s.The morphology,sustained release of silver ions,biocompatibility and antibacterial properties of the hydrogels were characterized by SEM,ICP,MTT assay and spread plate method,separately.The experimental results indicated that polyaspartic-acid-based hydrogel formed with PEI-AgNCs exhibited better biocompatibility towards L 929 cells and stronger antimicrobial activity against E.coli and S.aureus compared with the hydrogel formed with PEI.
作者
纪盈盈
张瑜
张洪禹
何盼
JI Yingying;ZHANG Yu;ZHANG Hongyu;HE Pan(School of Materials Science and Engineering,Changchun University of Science and Technology,Changchun 130022,China;Key Laboratory of Polymer Ecomaterials,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China)
出处
《功能材料》
北大核心
2025年第7期7146-7152,共7页
Journal of Functional Materials
基金
国家自然科学基金项目(51803014,52273310)
吉林省自然科学基金项目(20240101113JC)。
关键词
聚天冬氨酸
抗菌水凝胶
纳米银
聚乙烯亚胺
polyaspartic acid
antibacterial hydrogel
nano-silver
polyethyleneimine
