摘要
目的通过阴道松弛症患者阴道菌群组成特征、与非松弛症人群的菌群分布及功能差异,探究阴道菌群与阴道松弛症的相关性,为阴道松弛症的诊治提供新思路。方法选取2024年5月至11月在北京大学人民医院就诊的阴道松弛症患者30例和无阴道松弛症女性(对照组)30例,采集其阴道分泌物样本,利用细菌16S rDNA扩增技术提取阴道菌群基因组DNA,并使用生物信息学软件进行数据处理,比较两组阴道菌群的组成、分布及基因功能差异。结果研究组中以普雷沃氏菌属、阿托比菌属和厌氧球菌属为主;对照组中以乳杆菌属为主,链球菌属略高于研究组。两组α-多样性(P>0.05);Rank Abundance曲线整体趋势高度重合,β多样性上存在显著分离(Anosim R=0.117,P=0.01;Adonis R^(2)=0.072,P=0.015)。结合LEf Se分析与ROC曲线筛选出标识性菌属如下:嗜胨菌属(AUC=0.74)、细金氏菌属(AUC=0.72)、普雷沃氏菌属(AUC=0.71)、卟啉单胞菌属(AUC=0.70)、阿托比菌属(AUC=0.69)、棒状杆菌属(AUC=0.69)。两组间存在多条具有显著表达差异(P<0.05)的代谢通路与酶合成基因表达通路。结论阴道松弛症与阴道菌群具有相关性。
Objective By investigating the compositional characteristics of vaginal microbiome in vaginal laxity patients and the distributional/functional differences compared with non-laxity populations,this study explores the correlation between vaginal microbiome and vaginal laxity,providing novel insights for its diagnosis and treatment.Methods A total of 30 vaginal laxity reproductive-aged patients(case group)and 30 non-laxity women(control group)were recruited from Peking University People's Hospital between May and November 2024.Vaginal secretion samples were collected,and bacterial 16S rDNA amplification technology was used to extract vaginal microbiome genomic DNA.Bioinformatics software was employed for data processing to compare the composition,distribution,and genetic function differences of vaginal microbiome between the two groups.Results The case group was predominantly composed of Prevotella,Atopobium,and Anaerococcus,while the control group showed Lactobacillus dominance with slightly higher Streptococcus abundance compared to cases.No significant α-diversity differences were observed(P>0.05),with highly overlapping Rank Abundance curves.β-diversity analysis demonstrated significant separation(Anosim R=0.117,P=0.01;Adonis R^(2)=0.072,P=0.015).LEfSe-ROC integrated analysis identified biomarker genera:Peptoniphilus(AUC=0.74),Finegoldia(AUC=0.72),Prevotella(AUC=0.71),Porphyromonas(AUC=0.70),Atopobium(AUC=0.69),and Corynebacterium(AUC=0.69).Significant differential expression(P<0.05)was detected in multiple metabolic pathways and enzymatic synthesis gene pathways between groups.ConclusionssVaginal laxity in reproductive-aged women is correlated with vaginal microbiome composition.
作者
赵悦欣
李文燕
魏小停
王世言
谢冰
孙秀丽
ZHAO Yuexin;LI Wenyan;WEI Xiaoting;WANG Shiyan;XIE Bing;SUN Xiuli(Department of Obstetrics and Gynecology,Peking University People's Hospital,Beijing 100044,China)
出处
《中国妇产科临床杂志》
北大核心
2025年第4期325-329,共5页
Chinese Journal of Clinical Obstetrics and Gynecology
基金
北京市通州区科技创新人才项目(JCQN2024008)
北京市自然科学基金-海淀原始创新联合基金资助项目(L232076)。
