摘要
碱性亮氨酸拉链(bZIP)转录因子是植物中最大的转录因子家族之一,广泛参与了植物生长发育、胁迫响应和次生代谢产物生物合成等过程。本研究在党参(Codonopsis pilosula)基因组中鉴定出41个bZIP家族基因。系统发育分析将其分为13个亚族;基因结构和保守基序分析表明,同一亚族的蛋白具有相似的保守基序和结构域,Motif 1为bZIP家族特有的碱性区域,保守性最强,在党参bZIP蛋白中均有分布;顺式作用元件分析表明,bZIP成员上游启动子区域存在多种非生物胁迫元件、激素响应元件和防御应激反应元件,其中光信号响应元件占比最大;转录组表达模式分析显示,17条bZIP基因在高光处理下表达量升高,其中的6条积极响应光信号。本研究将为党参bZIP转录因子的功能研究奠定基础,为参与光信号响应的bZIP转录因子的筛选提供依据。
The basic leucine zipper(bziP)transcription factors represent one of the largest transcription factor families in plants,playing crucial regulatory roles in growth and development,stress responses,and secondary metabolite biosynthesis.In this study,we identified 41 bZiP family genes in Codonopsis pilosula.Phylogenetic analysis classified these genes into 13 distinct subfamilies.Gene structure and conserved motif analyses revealed that proteins within the same subfamily share similar motif compositions and domain architectures.Notably,Motif 1,corresponding to the characteristic bziP basic region,exhibited the highest conservation and was present in all C.pilosula bziP proteins.The cis-acting regulatory elements analysis identified numerous stress-responsive,hormone-responsive,and defense-related elements in the promoter regions of bziP genes,with light-responsive elements being the most abundant.Transcriptome profiling demonstrated that 17 bZiP genes were upregulated under high-light conditions,among which 6 showed particularly strong responsiveness to light signals.This study provides a fundamental basis for functional research on bziP transcription factors in C.pilosula and offers critical references for screening light-responsive bziP transcription factors.
作者
张晨
刘吉爽
姚远
肖莹
ZHANG Chen;LIU Jishuang;YAO Yuan;XIAO Ying(State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《植物生理学报》
北大核心
2025年第6期777-788,共12页
Plant Physiology Journal
基金
国家重点研发计划(2022YFC3501700和2023YFC3504800)
国家自然科学基金(32170402)
上海市优秀学术带头人项目(23XD-1423500)
上海中医药大学有组织科研专项(2023YZZ01)。
关键词
bZIP转录因子
党参
系统发育分析
光信号响应
bZiP transcription factor
Codonopsis pilosula
phylogenetic analysis
light signal response
