摘要
目的:研究褐藻胶寡糖(alginate oligosaccharides,AOS)对葡聚糖硫酸钠(dextran sulfate sodium,DSS)诱导的小鼠炎症性肠病的影响,并通过AOS对肠道菌群和短链脂肪酸的影响探讨相关的可能机制。方法:雄性C57BL/6小鼠饮用5 d 1.5%DSS溶液进行急性结肠炎建模,同时以0.5、1 g/kg和1.5 g/kg的梯度剂量口服9 d AOS。在第10天评估体质量、结肠长度、疾病活动指数以及组织损伤。使用聚合酶链式反应法评估结肠中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、核转录因子-κB(nuclear factor kappa B,NF-κB)、白细胞介素-6(interleukin-6,IL-6)、IL-1β、紧密连接蛋白(zonal occludens-1,ZO-1)和G蛋白偶联受体43(G protein-coupled receptor43,GPR43)的mRNA表达水平。采用高效液相色谱测定短链脂肪酸含量并分别对微生物群落进行测序。结果:AOS显著减轻了结肠炎症状,如体质量下降、结肠缩短、组织损伤,并降低了TNF-α、NF-κB、IL-6和IL-1β的mRNA表达,增加了ZO-1和GPR43的mRNA表达。且AOS可显著增加丙酸杆菌的数量,同时抑制肠球菌和放线菌的生长;高剂量AOS还可以促进乳酸、乙酸和丙酸的产生。结论:AOS对DSS诱导的小鼠结肠炎具有积极的改善作用,其作用机制可能与肠道菌群和短链脂肪酸的调节有关,具有作为改善肠道炎症的功能性食品的潜力。
Objective:To investigate the effect of alginate oligosaccharides(AOS)on dextran sulfate sodium(DSS)-induced inflammatory bowel disease(IBD)in mice and to explore the possible mechanism based on the effect of AOS on the intestinal flora and short-chain fatty acids(SCFAs).Methods:Male C57BL/6 mice were provided with 1.5%DSS for 5 days for acute colitis modeling.Meanwhile,mice were orally administrated with gradient doses of AOS(0.5,1,and 1.5 g/kg)for 9 days.On day 10,body mass,colon length,disease activity index(DAI),and tissue damage were assessed.Polymerase chain reaction was used to assess the mRNA expression levels of tumor necrosis factor-α(TNF-α),nuclear factor kappa B(NF-κB),interleukin-6(IL-6),IL-1β,the tight junction protein zonula occluden-1(ZO-1)and G protein-coupled receptor 43(GPR43).SCFAs production was measured by high performance liquid chromatography(HPLC)and the microbial community was analyzed by high-throughput sequencing.Results:AOS significantly alleviated the symptoms of colitis,such as body mass loss,colon shortening,and tissue damage,and reduced the mRNA expression of TNF-α,NF-κB,IL-6 and IL-1β,while increasing the expression levels of ZO-1 and GPR43.Additionally,AOS significantly increased the number of Propionibacterium,while inhibiting the growth of Enterococcus and Actinomyces;high-dose AOS also enhanced the production of colonic lactate,acetate,and propionate.Conclusion:AOS play a positive role in ameliorating DSS-induced colitis in mice,and the effect may be associated with the regulation of the intestinal flora and SCFAs.Hence,AOS have the potential as a functional food ingredient to alleviate intestinal inflammation.
作者
任晓敏
秦明珍
张少龙
张帆
严芬
REN Xiaomin;QIN Mingzhen;ZHANG Shaolong;ZHANG Fan;YAN Fen(College of Biological Science and Engineering,Fuzhou University,Fuzhou 350108,China)
出处
《食品科学》
北大核心
2025年第15期224-231,共8页
Food Science
基金
福建省科技厅引导性项目(2024N0001)
福建省财政厅项目(闽财指[2024]900号)。
关键词
褐藻胶寡糖
炎症性肠病
肠道菌群
短链脂肪酸
alginate oligosaccharides
inflammatory bowel disease
intestinal flora
short-chain fatty acids
