摘要
探究动物双歧杆菌J12及其后生元对环磷酰胺(cyclophosphamide,CTX)干扰小鼠免疫功能的调节作用和肠道菌群结构的影响。结果表明动物双歧杆菌J12活细胞能调节脾脏T细胞、自然杀伤细胞、自然杀伤T细胞、细胞毒性T淋巴细胞和辅助性T(T helper,Th)细胞数量,J12发酵上清液可显著缓解CTX对Th细胞的抑制作用,但J12热灭活死细胞对CTX诱导的免疫抑制没有表现出显著的改善效果。J12活细胞及发酵上清液可以改善CTX诱导的小鼠肠道菌群失衡,显著增加肠道中Alloprevotella的丰度,减少Lachnospiraceae_NK4A136_group的丰度,进而调节代谢和免疫系统,恢复紊乱的免疫状态。综上所述,J12活细胞可以有效地加速CTX诱导的免疫抑制的恢复,可作为对抗免疫抑制的潜在免疫调节剂。
This study explored the regulatory effects of Bifidobacterium animalis J12 and its postbiotics on immunosuppression induced by cyclophosphamide(CTX)in mice and the impact on the structure of gut microbiota.The results indicated that the live cells of B.animalis J12 regulated the number and overexpression of T,natural killer(NK),natural killer T(NKT),cytotoxic T(CTL),and T helper(Th)lymphocytes in the spleen of CTX immunosuppressed mice.The fermentation supernatant significantly alleviated the impact of CTX on Th cells,but the heat-inactivated cells of J12 did not show significant effects on CTX-induced immunosuppression.Both the live cells and fermentation supernatant rescued gut microbiota imbalance induced by CTX,significantly increasing the abundance of Alloprevotella in the gut,and reducing the abundance of Lachnospiraceae_NK4A136_group,thereby regulating the metabolism and immune system,and restoring the disordered immune state.In summary,J12 live cells can effectively accelerate recovery from immunosuppression induced by CTX,indicating its potential as an immunomodulator.
作者
张静
梁启萌
宋晓东
金君华
谢远红
张红星
ZHANG Jing;LIANG Qimeng;SONG Xiaodong;JIN Junhua;XIE Yuanhong;ZHANG Hongxing(Beijing Laboratory of Food Quality and Safety,Food Science and Engineering College,Beijing University of Agriculture,Beijing 102206 China;Inner Mongolia Mengniu Dairy(Group)Co.Ltd.,State Key Laboratory of Dairy Quality Digital Intelligence Monitoring Technology,State Administration for Market Regulation,Hohhot 011517,China)
出处
《食品科学》
北大核心
2025年第15期174-183,共10页
Food Science
基金
“十四五”柳州市重点研发计划项目(2025SB0603A001)。
关键词
热灭活死细胞
活细胞
双歧杆菌
免疫
环磷酰胺
heat-inactivated cells
live cells
Bifidobacterium
immunity
cyclophosphamide
