摘要
旨在探究乳酸菌(lactic acid bacteria,LAB)对黄羽肉鸡生长性能、消化功能和养分利用率的影响。选取480只1日龄(31.95±0.56)g雄性黄羽肉仔鸡,随机分为3组,每组8个重复,每个重复20只鸡,试验期为70 d,分为前期(1~35 d)和后期(36~70 d)两个阶段。对照组肉鸡饲喂基础饲粮(CON组),试验组(LAB1、LAB2组)肉鸡饲喂分别在基础饲粮中添加500、1000 mg·kg^(-1)乳酸菌(活菌数1×10~8 CFU·g-1)的试验饲粮。结果表明:LAB1和LAB2组肉鸡35 d肌胃和胰腺指数、70 d的胰腺指数、33~35 d和68~70 d有机物表观代谢率以及68~70 d粗蛋白质表观代谢率均高于CON组,35 d肉鸡血清尿素氮含量均低于CON组(P<0.05);LAB2组的肉鸡末重、日增重和日采食量均高于CON组,68~70 d粗蛋白质表观代谢率高于CON组和LAB1组(P<0.05)。LAB1和LAB2组肉鸡的胰腺35 d脂肪酶、空肠35 d淀粉酶、脂肪酶活性和空肠70 d胰蛋白酶、淀粉酶和脂肪酶活性、胰腺35和70 d胰蛋白酶基因表达量以及70 d淀粉酶基因表达量均高于CON组;LAB1组肉鸡的35 d空肠胰蛋白酶活性高于CON组和LAB2组,LAB2组的胰腺70 d胰蛋白酶活性和空肠70 d淀粉酶、胰蛋白酶活性高于CON组和LAB1组(P<0.05)。综上所述,饲粮中添加乳酸菌可上调胰腺消化酶基因表达量,增加胰腺和空肠食糜消化酶活性,降低血清尿素氮含量,提高肉鸡有机物和粗蛋白质表观代谢率,改善肉鸡消化器官发育和生长性能,在本试验条件下,1000 mg·kg^(-1)乳酸菌添加剂量效果更佳。
The purpose of this study was to investigate the effects of lactic acid bacteria(LAB)on the growth performance,digestive function,and nutrient utilization of yellow-feathered chickens.A total of 480 male yellow-feathered broiler chickens with an initial body weight of(31.95±0.56)g were randomly divided into 3 groups,with 8 replicates per group and 20 chickens per replicate.The experiment lasted for 70 days,which was divided into an early phase(1-35 days)and a late phase(36-70 days).The broiler chickens in the control group(CON)were fed a basal diet,while the experimental groups(LAB1 and LAB2)were fed diets supplemented with 500 mg·kg^(-1) or 1000 mg·kg^(-1) of LAB(with a live bacteria count of 1×108 CFU·g-1),respectively.The results showed that broiler chickens in the LAB1 and LAB2 groups had higher gizzard and pancreas indices at 35 days of age,higher pancreas indices at 70 days of age,higher apparent metabolic rates of organic matter at 33-35 and 68-77 days of age and crude protein at 68-70 days of age,and lower serum urea nitrogen content at 35 days of age compared to those in CON group(P<0.05).The broiler chickens in the LAB2 group exhibited significantly higher final body weight,average daily weight gain,average daily feed intake,and apparent metabolic rate of crude protein at 68-70 days of age than those of both the CON and LAB1 groups(P<0.05).The LAB1 and LAB2 groups showed higher lipase activity in the pancreas at 35 days of age,higher amylase and lipase activities in the jejunal chyme at 35 days of age,higher trypsin,amylase,and lipase activities in the jejunal chyme at 70 days of age,and higher expression of Trypsin genes at 35 and 70 days as well as Amylase genes at 70 days in the pancreas compared to the CON group.The LAB1 group had higher trypsin activity in the jejunal chyme at 35 days of age than the CON and LAB2 groups,and the LAB2 group had higher trypsin activity at 70 days of age in the pancreas and higher amylase and trypsin activities in the jejunal chyme at 70 days of age compared to the CON and LAB1 groups(P<0.05).In conclusion,dietary supplementation with LAB upregulated the expression of digestive enzyme genes,increased the activity of digestive enzymes in the pancreas and jejunal chyme,reduced serum urea nitrogen content,improved the apparent metabolic rates of organic matter and crude protein,enhanced digestive organ development,and improved the growth performance in broiler chickens.The effects were more pronounced with the 1000 mg·kg^(-1) LAB supplementation under the condition of the current experiment.
作者
孙淑佳
郑嘉祺
卢姝婉
刘金松
姚春雷
杨彩梅
许英蕾
张瑞强
SUN Shujia;ZHENG Jiaqi;LU Shuwan;LIU Jinsong;YAO Chunlei;YANG Caimei;XU Yinglei;ZHANG Ruiqiang(College of Animal Science and Technology,College of Veterinary Medicine,Zhejiang A&F University,Hangzhou 311300,China;Zhejiang Huijia Bio-technology Co.,Ltd.,Huzhou 313300,China;Haiyan Animal Husbandry and Veterinary Service Center,Jiaxing 314300,China)
出处
《畜牧兽医学报》
北大核心
2025年第7期3335-3343,共9页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
浙江省重点研发计划项目(2024C04016)
海盐县科技计划项目(2024ND04)
浙江省重点农业企业研究院项目(2021Y30004)。
关键词
乳酸菌
黄羽肉鸡
生长性能
表观代谢率
消化酶活性
lactic acid bacteria
yellow-feathered broiler
growth performance
nutrient apparent metabolic rate
digestive enzyme activity
