摘要
为分析天麻中多酚的物质组成及其降血糖活性,本研究利用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱联用技术对纯化后的天麻多酚进行成分鉴定,同时通过体外实验分析天麻多酚对α-淀粉酶和α-葡萄糖苷酶活性的抑制作用,并探讨天麻多酚降血糖的作用机制。结果表明:天麻多酚含有30种化合物,包含黄酮类化合物23种、酚酸类化合物2种和酚类化合物5种;体外降血糖实验结果显示,天麻多酚对α-淀粉酶和α-葡萄糖苷酶均有较强的抑制作用,且抑制率和天麻多酚浓度呈正相关关系,当天麻多酚质量浓度为0.5 mg/mL时对α-淀粉酶和α-葡萄糖苷酶的抑制率分别为(79.71±2.51)%和(77.33±2.31)%,其半抑制质量浓度分别为(0.057±0.011)mg/mL和(0.189±0.017)mg/mL,且对二者的抑制类型分别为反竞争性-非竞争性混合抑制和竞争性-非竞争性混合抑制。本研究结果可为进一步明晰天麻多酚的降血糖机制及其资源的开发利用提供一定的理论依据。
To investigate the composition and hypoglycemic activity of polyphenols in Gastrodia elata,this study utilized ultra-high performance liquid chromatography coupled with quadrupole and electrostatic field Orbitrap high-resolution mass spectrometry(UPLC-Q-Exactive Orbitrap-MS)to identify the components of the purified polyphenols.In vitro experiments were conducted to evaluate the inhibitory effects of G.elata polyphenols onα-amylase andα-glucosidase activities and to explore the mechanism underlying their hypoglycemic effects.The results indicated that G.elata polyphenols contained 30 compounds,including 23 flavonoids,2 phenolic acids,and 5 phenolic compounds.In vitro hypoglycemic experiments showed that G.elata polyphenols exhibited significantly inhibitory effects on bothα-amylase andα-glucosidase,which were positively correlated with polyphenol concentration.At a concentration of 0.5 mg/mL,the inhibitory rates of G.elata polyphenols onα-amylase andα-glucosidase were(79.71±2.51)%and(77.33±2.31)%,respectively.The half-maximal inhibitory concentrations(IC50)were(0.057±0.011)and(0.189±0.017)mg/mL,respectively.The types of inhibition were determined to be mixed uncompetitive-noncompetitive inhibition forα-amylase and mixed competitive-noncompetitive inhibition forα-glucosidase.These findings could provide a theoretical foundation for further research into the hypoglycemic mechanisms of G.elata polyphenols and for their development and utilization.
作者
董仕豪
徐柠檬
付正建
申开泽
曾顺超
范方宇
郭磊
DONG Shihao;XU Ningmeng;FU Zhengjian;SHEN Kaize;ZENG Shunchao;FAN Fangyu;GUO Lei(College of Biological Science and Food Engineering,Southwest Forestry University,Kunming 650224,China;Yunnan Key Laboratory of Gastrodia and Fungi Symbiotic Biology,Zhaotong University,Zhaotong 657000,China;Shuangjiang Rongshengyuan Food Co.,Ltd.,Lincang 677300,China)
出处
《食品科学》
北大核心
2025年第16期285-294,共10页
Food Science
基金
云南省天麻与真菌共生生物学重点实验室开放课题(TMKF2024B05)
云南省科技厅基础研究专项(202101AT070042)。
关键词
天麻
多酚
成分鉴定
Α-淀粉酶
Α-葡萄糖苷酶
抑制作用
Gastrodia elata
polyphenols
composition identification
α-amylase
α-glucosidase
inhibitory effect
