摘要
为探究Ⅰ群4型禽腺病毒(FAdV-4)卵黄抗体中和效价与预防效果、治疗效果的相关性,对制备的卵黄抗体进行2^(-1)~2^(-4)梯度稀释(中和效价分别为1:282、1:142、1:79、1:36)后,分别进行预防和治疗效果试验。结果显示,在FAdV-4卵黄抗体中和效价不低于1:142时,分别进行预防后攻毒和攻毒后治疗试验,发现试验鸡健活率均不低于80%。结果表明,预防和治疗效果与卵黄抗体效价呈正相关。为保证卵黄抗体的临床使用效果,建议将抗体成品效力检验的中和效价标准定为不低于1:256。本研究为FAdV-4卵黄抗体的临床应用剂量选择提供了数据支持,为卵黄抗体质量标准的制定奠定了理论基础。
In order to investigate the correlation between neutralization antibody titer of adenovirus serotype 4(FAdV-4)under group I and prevention and treatment effects against the virus,the prepared egg-yolk antibodies were diluted at a gradient of 2^(-1)-2^(-4)(neutralization titers were 1:282,1:142,1:79 and 1:36,respectively)to test their prevention and treatment effects,respectively.It was found that the survival rates of the chickens tested were not less than 80%after post-prevention challenge and post-challenge treatment tests when the titer was not lower than 1:142.In conclusion,the prevention and treatment effects were positively correlated with the egg-yolk antibody titer.For the purpose of ensuring the clinical effect of the antibodies,the standard titer should be set at no less than 1:256.In the study,data was provided to select clinical dose of FAdV-4 yolk antibodies,and a theoretical foundation to develop the quality standard of yolk antibodies.
作者
蔡青秀
孙亚磊
于春梅
丛雁方
董照洋
李宸辉
戴建君
Cai Qingxiu;Sun Yalei;Yu Chunmei;Cong Yanfang;Dong Zhaoyang;Li Chenhui;Dai Jianjun(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China;Qingdao Vland Biotech INC/National Engineering and Technology Research Center for Animal Health Products,Qingdao 266109,Shandong,China)
出处
《中国动物检疫》
2025年第4期106-111,共6页
China Animal Health Inspection
基金
青岛市科技惠民示范专项(24-1-8-xdny-21-nsh)。
关键词
Ⅰ群4型禽腺病毒卵黄抗体
预防效果
治疗效果
相关性
egg-yolk antibodies against FAdV-4 under group I
prevention effect
treatment effect
correlation
