摘要
为探究黄连“气、色、味、量”相关性,及品种、产地对其感官特征与成分的影响。本研究采用电子仿生技术测定黄连的气味、味道、颜色值,HPLC测定生物碱含量,UPLC-Q-TOF-MS/MS鉴定化合物成分,以SPSS 22.0分析黄连“气、色、味、量”相关性,以正交偏最小二乘判别分析(orthogonal partial least squares discriminant analysis,OPLS-DA)、偏最小二乘法判别分析(partial least squares discriminant analysis,PLS-DA)鉴别黄连品种以及产地。结果表明,黄连“气、色、味、量”主要响应值呈显著正相关。在黄连品种鉴别中,味连的气味、味道值明显高于云连、雅连,HPLC分析显示云连中小檗碱含量高于雅连、味连,UPLC-Q-TOF-MS/MS鉴定出味连的化学成分明显高于云连、雅连,OPLS-DA模型很好鉴别黄连品种,且颜色b*值的影响最大;在味连产地鉴别中,湖北产的“气、味”值高,川鄂产的颜色和小檗碱含量高,以PLS-DA模型区分味连产地发现颜色产地区分影响最大。本研究实现黄连感官特征数字化鉴别,为品种与产地区分提供依据。
To investigate the correlations between the odor,color,taste,and alkaloid content of Coptidis Rhizoma,and the influence of cultivars and origins on its sensory characteristics and chemical composition,this research used electronic bionic technology to determine the odor,taste,and colorimetric values of Coptidis Rhizoma,and HPLC was used to determine alkaloid content,while UPLC-Q-TOF-MS/MS was applied to identify chemical components.SPSS 22.0 was utilized to analyze correlations among the odor,color,taste,and alkaloid content parameters.Orthogonal partial least squares discriminant analysis(OPLS-DA)and partial least squares discriminant analysis(PLS-DA)were used to identify the cultivars and origins of Coptidis Rhizoma.The research showed that there were significant positive correlations among these aspects.In the cultivars identification of Coptidis Rhizoma,Coptis chinensis rhizomes(CCR)had higher odor and taste values than C.teeta rhizomes(CTR)and C.deltoidea rhizomes(CDR).HPLC analysis indicated higher berberine hydrochloride content in CTR than in CDR and CCR.UPLC-Q-TOF-MS/MS analysis revealed that CCR had more chemical components than CTR and CDR.The OPLS-DA model effectively distinguished Coptidis Rhizoma cultivars,and the color b*value was the key discriminative factor.In origin identification of CCR,odor and taste values of CCR from Hubei were higher than those from Sichuan and Chongqing,color value and berberine hydrochloride content of CCR in Sichuan and Hubei were higher than in Chongqing.The PLS-DA model could distinguish the origins well of CCR,the PLS-DA model for origin identification demonstrated that color was the most important factor.This research successfully accomplished the digital discrimination of the sensory characteristics of Coptidis Rhizoma,providing a basis for the differentiation of cultivars and origins.
作者
冉继春
张小梅
阳勇
潘舒湘
李舒雅
何丹
RAN Ji-chun;ZHANG Xiao-mei;YANG Yong;PAN Shu-xiang;LI Shu-ya;HE Dan(School of Pharmacy,Chongqing Medical University,Chongqing 400016,China;Chongqing Academy of Chinese Materia Medica;Sichuan-Chongqing Joint Key Laboratory of Innovation of New Drugs of Traditional Chinese Medicine,Chongqing 400065,China)
出处
《天然产物研究与开发》
北大核心
2025年第7期1267-1278,共12页
Natural Product Research and Development
基金
重庆市自然科学基金面上项目(cstc2020jcyj-msxmX0534)
重庆市绩效激励引导专项(jxjl20210004)
重庆市中医药传承创新团队项目(2023)。
关键词
黄连
电子仿生技术
品种
产地
鉴别
Coptidis Rhizoma
electronic bionic technology
cultivars
origin
identification
