摘要
2021—2023年,在福建省福州市的无花果果园发现细菌性叶斑病,田间株发病率达50%以上。为明确病因,采集病叶进行实验室菌株分离,经柯赫氏法则验证其致病性,并综合细菌的生物学特征、生理生化反应、多基因序列分析明确其分类地位。结果表明,从采集的病叶中分离纯化获得6株细菌菌株,经柯赫氏法则验证其为病原菌。菌株在NA培养基平板上的菌落为乳白色,圆形,微隆起。细菌菌体呈短杆状,极生1~6根鞭毛,革兰氏染色阴性。对菌株开展的27项生理生化测定结果与对照菌株——丁香假单胞菌(Pseudomonas syringae)DC3000菌株一致,在金氏B(KB)培养基平板上可产生绿色水溶性荧光色素,过氧化氢酶反应和淀粉水解的测试结果为阳性,精氨酸双水解、硝酸钾还原反应、吲哚反应、果聚糖利用和氧化酶反应的测试结果为阴性,可利用的碳水化合物为葡萄糖、甘露醇、丙二酸钠和丙三醇。基于16S rDNA序列,及gyrB、rpoD和cts的基因构建系统发育树,供试菌株与丁香假单胞菌丁香致病变种(P.syringae pv.syringae)聚为一支。综上,将分离到的细菌菌株鉴定为丁香假单胞菌丁香致病变种。这是国内首次发现丁香假单胞菌丁香致病变种在自然状态下侵染无花果并引起叶斑病。
From 2021 to 2023,a new bacterial leaf spot disease on Ficus carica was found in Fuzhou,Fujian Province,China,with an incidence above 50%.To clarify the cause of the disease,symptomatic leaf samples were collected and taken back to the laboratory to isolate the bacteria.The pathogenicity of the isolated strains were determined via Koch's rule,and its taxonomic status was confirmed based on the bacterial biological characteristics,physiological and biochemical characters,and multi-gene phylogenetic analysis.The results showed that 6 bacterial strains were obtained by isolating and purifying from the diseased leaves,and all the bacterial strains could cause the same disease on Ficus carica.The bacterial colonies were round and bulged with milk white.The bacterial cells were rod-shaped and had 1-6 flagella on polar.The Gram staining reaction was negative.The test results regarding 27 biological and biochemical characters of the isolated strains were consistent with Pseudomonas syringae strain DC3000.The bacterial cells could produce green water-soluble fluorescent on King's B medium.They were capable of hydrolyzing starch and had hydrogen peroxide activities.Meanwhile,they were not capable of potassium nitrate reduction,arginine hydrolysis,indole production or fructan utilization,and had no oxidase activities.They could utilize glucose,mannitol,sodium malonate,glycerol as carbon sources.As revealed by the constructed phylogenetic trees based on the sequences of 16S rDNA,and gyrB,rpoD,cts genes,the isolated strains were clustered with P.syringae pv.syringae.Above all,the isolated bacterial strains were identified as P.syringae pv.syringae.This was the first report that P.syringae pv.syringae could naturally cause leaf spot disease on Ficus carica in China.
作者
季梦婷
陈长江
朱玲
詹梦琳
肖顺
蔡学清
JI Mengting;CHEN Changjiang;ZHU Ling;ZHAN Menglin;XIAO Shun;CAI Xueqing(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Computer and Information Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Tianhe Lyubao Agricultural Materials Co.,Ltd.,Fuzhou 350193,China)
出处
《浙江农业学报》
北大核心
2025年第5期1097-1106,共10页
Acta Agriculturae Zhejiangensis
基金
福建省现代农业(水果)产业技术体系植保与土肥岗位(闽农科教[2023]16号)
福建省科技特派员工作经费(KTP19128A,KTP21267A)。
关键词
无花果细菌性叶斑病
病原菌鉴定
丁香假单胞菌丁香致病变种
Ficus carica bacterial leaf spot disease
pathogen identification
Pseudomonas syringae pv.Syringae
