摘要
【目的】探究黑木耳不同颜色子实体代谢物差异,为后续黑木耳颜色差异研究和产品的开发利用提供参考依据。【方法】基于高效液相色谱-质谱联用技术对同一品种黑木耳不同颜色子实体进行非靶向代谢组学研究。【结果】在黑木耳黄色(HY)和黑色(HH)子实体中共鉴定到1624个代谢产物。HY和HH相比共有372个显著差异代谢物,其中347个上调,25个下调。差异代谢物主要分为氨基酸及短肽、莽草酸及苯丙酸、生物碱、萜类等物质,其中萜类化合物数量最多(91个)。此外,黑木耳子实体中相对含量较高的是脂肪酸和氨基酸及短肽类物质,且HY中的相对含量显著高于HH。显著差异代谢物主要富集在氨基酸生物合成、ABC转运蛋白等通路,其中氨基酸生物合成途径富集最显著。17种常见氨基酸中除苯丙氨酸外,其余在HY中的相对含量均显著高于HH,酪氨酸最突出,高约21倍。【结论】氨基酸及短肽、莽草酸及苯丙酸、生物碱、萜类等物质在黑木耳不同颜色子实体中差异显著,可能是影响颜色形成的物质基础。
[Objective]The study aimed to investigate the metabolite disparity in fruiting bodies of Auricularia heimuer with different colors,and to provide a reference for the subsequent research on the color difference of A.heimuer and the development and utilization of products.[Method]Untargeted metabolomics of the fruiting bodies with different colors from a same variety was studied by high performance liquid chromatography-mass spectrometry.[Result]A total of 1624 metabolites were identified in both yellow(HY)and black(HH)fruiting bodies.A total of 372 metabolites exhibited significant differences,with 347 up-regulated and 25 down-regulated.These varied metabolites encompassed amino acids and peptides,shikimates and phenylpropanoids,alkaloids,terpenoids,etc.,with terpenoids being the predominant group(91).Notably,the fruiting bodies of A.heimuer contained the highest levels of faty acids,amino acids and peptides,with HY exhibiting notably higher values compared to HH.Different metabolites were predominantly enriched in biosynthesis of amino acids,ABC transporter and other pathways,with biosynthesis of amino acids pathway predominantly.Additionally,the relative content of common amino acids except phenylalanine was significantly higher in HY than that in HH,with tyrosine standing out as the most notable.[Conclusion]Amino acids and peptides,shikimates and phenylpropanoids,alkaloids,terpenoids are significantly different in different color fruiting bodies of A.heimuer,which may be the material basis affecting the formation of color.
作者
刘询
李通
王晓巍
伏晓辉
贾定洪
何晓兰
王波
LIU Xun;LI Tong;WANG Xiao-wei;FU Xiao-hui;JIA Ding-hong;HE Xiao-lan;WANG Bo(Sichuan Academy of Agricultural Sciences/Sichuan Institute of Edible Fungi,Chengdu 610066,China;Gansu Academy of Agricultural Sciences,Lanzhou 730070,China;Tianshui Hong Ya He Mushroom Planting Co.,Ltd.,Tianshui,Gansu 741001,China)
出处
《西南农业学报》
北大核心
2025年第4期694-702,共9页
Southwest China Journal of Agricultural Sciences
基金
国家重点研发计划项目(2022YFD1602109)。
关键词
黑木耳
子实体
代谢物
差异分析
氨基酸
Auricularia heimuer
Fruiting body
Metabolites
Diference analysis
Amino acid
