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不同基质栽培黑木耳活性物质的靶向代谢组学分析 被引量:5

Targeted metabolomic analysis of active substances in Auricularia heimuer cultivated on different substrates
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摘要 黑木耳Auriculariaheimuer是一种重要的食药用菌,含有丰富的营养物质和生物活性成分,在我国被广泛栽培。分别以木屑(SW)、玉米芯(CO)和棉籽壳(CH)为主料栽培黑木耳,通过靶向代谢组学分析不同基质栽培的黑木耳子实体中糖、黄酮和酚酸类化合物以及必需氨基酸的含量差异。结果表明,SW基质栽培的黑木耳中糖类物质总量最低;总黄酮类物质含量无明显差异,仅牡荆素在CO栽培黑木耳中含量最高,CH栽培的最低;多酚类化合物在SW培养条件下的含量总体较高;而氨基酸及其衍生物的总量在CH栽培的子实体中最高,9种必需氨基酸的含量都明显高于SW和CO栽培组,尤其是具有重要生物学活性的赖氨酸和组氨酸差异最大。本研究全面呈现了不同基质栽培黑木耳子实体中糖类、黄酮类、多酚类化合物,以及必需氨基酸含量差异,研究结果为后续黑木耳营养品质鉴别和开发不同功效的功能性产品提供思路和依据。 Auricularia heimuer is an important edible and medicinal fungus,which contains rich nutrients and bioactive components,and is widely cultivated in China.Targeted metabolomics was used to examine the variations in sugar,flavonoid,polyphenol,and essential amino acid content in A.heimuer grown on sawdust(SW),corncob(CO),and cottonseed shell(CH).It was found that the lowest total sugar content was demonstrated in the fruiting bodies grown on SW substrate.There was no significant difference in total flavonoid content in the fruiting bodies grown on the different substrate.Vitexin content was the highest in the fruiting bodies grown on CO substrate,but the lowest in those grown on CH substrate.Polyphenol content was usually higher in the fruiting bodies grown on SW substrate.The total content of amino acids and their derivatives was the highest in the fruiting bodies on CH substrate,and the levels of nine essential amino acids,especially lysine and histidine having substantial biological activities,were much higher in fruiting bodies on CH substrate than in those on the SW and CO substrates.The comprehensive determination of the content of sugars,flavonoids,polyphenols and essential amino acids in the fruit bodies of A.heimuer cultivated in different substrates can provide clues and basis for identification of nutritional quality of A.heimuer and development of functional products with different functions.
作者 刘询 贾定洪 王晓巍 李通 王波 LIU Xun;JIA Dinghong;WANG Xiaowei;LI Tong;WANG Bo(Sichuan Institute of Edible Fungi,Sichuan Academy of Agricultural Sciences,Chengdu 610066,Sichuan,China;Gansu Academy of Agricultural Sciences,Lanzhou 730070,Gansu,China;Tianshui Hong Ya He Mushroom Planting Co.,Ltd.,Tianshui 741001,Gansu,China)
出处 《菌物学报》 CSCD 北大核心 2024年第12期76-88,共13页 Mycosystema
基金 国家重点研发计划(2022YFD1602109)。
关键词 黑木耳 代谢组学 栽培基质 活性物质 Auricularia heimuer metabolomics cultivation substrate active substance
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