摘要
将高温大曲与纯水以3∶10(g∶mL)混合、离心制取浸提液后,按照5∶100(g∶mL)接入高温大曲,基于摇瓶实验分别在40℃、45℃、50℃、55℃、60℃和65℃下对其进行培养,最后基于Illumina MiSeq高通量测序技术解析不同培养温度下样品中微生物的类群,并对比分析不同培养温度下样品中微生物群落的稳定性及功能。结果表明,45℃和50℃培养样品细菌菌群的丰富度和多样性均最高,而真菌菌群则在45℃培养样品中最高。随着培养温度的升高,样品中芽孢杆菌属(Bacillus)的相对含量逐渐增加,而片球菌属(Pediococcus)的相对含量逐渐降低;不同培养温度下样品中优势真菌菌群亦各不相同,在40℃、45℃、50℃和55℃培养样品的真菌菌群分别以毕赤酵母属(Pichia)、嗜热子囊菌属(Thermoascus)、曲霉菌属(Aspergillus)和复膜孢酵母属(Saccharomycopsis)为主,表明在使用某一特定温度对高温大曲源微生物进行培养时,虽会形成与该温度相对应的优势菌群,但其并未形成与该温度相对应的特有菌群。50℃和55℃培养样品中细菌和真菌菌群组成更丰富且相互作用关系更强。50℃培养样品中细菌菌群具有较高的防御功能(P<0.05)。
After mixing high-temperature Daqu with pure water at a ratio of 3∶10(g∶ml)and centrifuging to obtain the extract,it was inoculated into high-temperature Daqu at a ratio of 5∶100(g∶ml).Based on the shake flask experiment,it was cultivated at 40℃,45℃,50℃,55℃,60℃and 65℃respectively.The microbial groups in samples at different culture temperatures were analyzed based on Illumina MiSeq high-throughput sequencing technology,and the stability and function of microbial communities in samples at different culture temperatures were compared and analyzed.The results showed that the richness and diversity of the bacterial flora in the samples cultured at 45℃and 50℃were the highest,while the fungal flora was the highest in the samples cultured at 45℃.With the increase of the culture temperature,the relative content of Bacillus in the sample gradually increased,while the relative content of Pediococcus gradually decreased.The dominant fungal flora in the samples also varied at different culture temperatures.The fungal communities of the samples cultured at 40℃,45℃,50℃and 55℃were mainly composed of Pichia,Thermoascus,Aspergillus and Saccharomycopsis,respectively.It indicated that when the high-temperature Daqu microorganisms were cultured at a certain specific temperature,although the dominant bacterial community corresponding to that temperature was formed,no specific bacterial community corresponding to that temperature was formed.The bacterial and fungal flora compositions in the samples cultured at 50℃and 55℃were more abundant and the interaction relationships were stronger.The bacterial flora in the samples cultured at 50℃had a relatively high defense function(P<0.05).
作者
赵恒
蔡文超
余培荣
陈炜
单春会
张海波
郭壮
ZHAO Heng;CAI Wenchao;YU Peirong;CHEN Wei;SHAN Chunhui;ZHANG Haibo;GUO Zhuang(Brewing Technology Industrial College,Hubei University of Arts and Sciences,Xiangyang 441053,China;Hubei ProvincialEngineering and Technology Research Center for Food Ingredients,Hubei University of Arts and Science,Xiangyang 441053,China;College of Food,Shihezi University,Shihezi 832000,China;Xinjiang Wuwu Liquor Industry Co.,Ltd.,Huyanghe 830099,China)
出处
《中国酿造》
北大核心
2025年第7期132-138,共7页
China Brewing
基金
新疆生产建设兵团第七师胡杨河市“揭榜挂帅”项目(QS2024011)。
关键词
温度
高温大曲
微生物类群
共现网络
基因功能
temperature
high-temperature Daqu
microbial group
co-occurrence network
gene function
