摘要
目的 观察大黄灵仙胶囊对胆管炎性模型大鼠胆管组织磷脂酰肌醇3-激酶(phosphatidylinositol 3-kinase, PI3K)、转化生长因子α(transforming growth factor alpha, TGF-α)、白细胞介素1β(interleukin-1 beta, IL-1β)表达的影响。方法 选择18只清洁级健康SD大鼠,按随机数字表法分成3组:假手术组、模型组、大黄灵仙组,每组6只。除假手术组外,其余两组均采用脂多糖(LPS)诱导构建大鼠胆管炎性动物模型,大黄灵仙组大鼠在制备胆管炎性模型前给予大黄灵仙胶囊颗粒溶液灌胃2 d,在制备胆管炎性模型后给予大黄灵仙胶囊颗粒溶液灌胃3 d,给药剂量为320 mg/(kg·d),假手术组、模型组大鼠每天给予2 mL/100 g蒸馏水灌胃,2次/d,连续干预5 d。处死各组大鼠,采用HE染色观察各组胆管病理形态改变,实时PCR法检测PI3K、TGF-α、IL-1β的mRNA表达,Western blot法检测PI3K、TGF-α、IL-1β的蛋白表达量。结果 HE染色显示采用大黄灵仙胶囊颗粒溶液灌胃大鼠的胆管组织病理炎性反应下降。与假手术组比较,模型组片内可见汇管区炎细胞浸润,个别区域可见肝细胞点状坏死,大鼠胆管组织的PI3K、TGF-α、IL-1β mRNA表达以及蛋白表达量均升高(P<0.05)。与模型组比较,大黄灵仙组大鼠片内可见汇管区少量炎细胞浸润,较模型组炎细胞减少,个别区域仍可见肝细胞点状坏死,炎细胞浸润面积显著减小,大黄灵仙组PI3K、TGF-α、IL-1β mRNA表达和蛋白表达量均降低(P<0.05)。结论 大黄灵仙胶囊可能通过调控胆管组织中PI3K、TGF-α、IL-1β表达,改善组织病理损害,最终发挥保护胆管组织的作用。
Objective To observe the effect of Dahuang Lingxian Capsules(大黄灵仙胶囊)on the expressions of phosphatidylinositol 3-kinase(PI3K),transforming growth factor alpha(TGF-α)and interleukin-1 beta(IL-1β)in bile duct tissues of rats with cholangioinflammation.Methods Eighteen clean-grade healthy SD rats were selected and divided into sham operation group,model group and Dahuang Lingxian Capsules group,with 6 rats in each group.Except for the sham operation group,the other two groups were induced by lipopolysaccharide(LPS)to construct a rat bile duct animal model.The rats in the Dahuang Lingxian Capsules group were given intragastric administration of Dahuang Lingxian Capsules Solution for 2 days before the preparation of the bile duct inflammation model,and the rats in the sham operation group and the model group were given 2 mL/100 g of distilled water intragastric administration twice a day for 5 consecutive days.The rats in each group were sacrificed,the pathological morphological changes of bile ducts in each group were observed by HE staining,the protein expressions of PI3K,TGF-αand IL-1βwere detected by Western blot,and the mRNA expressions of PI3K,TGF-αand IL-1βwere detected by real-time PCR.Results HE staining showed that the histopathological inflammatory response of the bile duct in rats administered with Dahuang Lingxian Capsules solution decreased.Compared with those in the sham operation group,inflammatory cell infiltration in the manifold area,punctate necrosis of hepatocytes in individual areas,and the mRNA and protein expressions of PI3K,TGF-αand IL-1βin bile duct tissues of rats were increased in the model group(P<0.05).Compared with those in the model group,a small number of inflammatory cells were infiltrated in the manifold area in the Dahuang Lingxian Capsules group,and the inflammatory cells significantly reduced compared with that of the model group.The hepatocytes were still punctate necrosis in some areas,the inflammatory cell infiltration area was significantly reduced,and the mRNA expressions and protein expressions of PI3K,TGF-αand IL-1βwere decreased(P<0.05).Conclusion Dahuang Lingxian Capsules may improve the histopathological damage by regulating the expressions of PI3K,TGF-αand IL-1βin bile duct tissue,and ultimately play a role in protecting bile duct tissue.
作者
黄文娟
于亚涛
林龙
王猛
陈雅璐
王清坚
HUANG Wenjuan;YU Yatao;LIN Long;WANG Meng;CHEN Yalu;WANG Qingjian(Guangxi University of Chinese Medicine,Nanning 530200,Guangxi,China;The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,Guangxi,China)
出处
《辽宁中医杂志》
北大核心
2025年第7期195-198,F0003,共5页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(82060867,82474507)
广西自然科学基金(2024GXNSFDA010025,2024GXNSFBA010106)
广西重点研发计划(桂科AB24010130)
王清坚广西名中医传承工作室建设项目(GZY2024011)。
