摘要
大鳞副泥鳅具有极高的营养价值,是朱鹮等多种濒危动物的主要食物来源。为评估大鳞副泥鳅作为饲料原料的安全性,揭示不同饲喂方式对大鳞副泥鳅代谢的影响,利用高效液相色谱-质谱(high performance liquid chromatography-mass spectrometry,HPLC-MS)技术对人工饲喂与天然采食两种不同饲喂方式下的大鳞副泥鳅进行了代谢组学分析。结果显示:大鳞副泥鳅营养物质丰富,共鉴定到680种化合物。人工饲喂商品化配合饲料显著改变了大鳞副泥鳅的代谢物组成,其中139种代谢物含量发生了显著改变,主要富集在脂质代谢途径。多种类固醇激素的含量,包括雌三醇、17α-炔雌醇和18-羟基皮质(甾)酮等雌激素,在人工饲喂的大鳞副泥鳅中显著增加(P<0.05);17α-甲基睾酮、1,4-雄烯二酮、16α-羟睾甾酮、11-酮本胆烷醇酮等雄激素含量则显著降低(P<0.05)。此外,γ-生育酚、亚油酸和α-亚麻酸等代谢物含量在人工饲喂的大鳞副泥鳅中也显著增加(P<0.05)。本研究从代谢水平初步解析了不同饲喂方式下大鳞副泥鳅的营养价值与食用安全性,为大鳞副泥鳅作为朱鹮等濒危动物饲料资源的开发与利用提供了理论依据。
Paramisgurnus dabryanus,commonly known as Dabry’s loach or large-scaled loach,has high nutritional value and serves as a main food source for endangered species such as crested ibises.In order to evaluate the safety of P.dabryanus as a feed source and investigate the effects of different feeding methods on its metabolism,metabolomic analysis was performed on Dabry’s loaches using high performance liquid chromatography-mass spectrometry(HPLC-MS).The results showed that Dabry’s loaches contained abundant nutrients,with a total of 680 identified compounds.Artificial feeding with commercial compound feed significantly altered the metabolic profile of Dabry’s loaches,with 139 metabolites showing significant changes,mainly enriched in lipid metabolism pathways.Several steroid hormones including estrogens(estriol,17琢-ethinylestradiol,and 18-hydroxycorticosterone)were found to accumulate significantly in the artificially fed Dabry’s loaches,while the levels of male hormones,such as 17琢-methyltestosterone,1,4-androstanedione,16琢-hydroxytestosterone,and 11-ketotestosterone,were significantly reduced(P<0.05).In addition,the levels of酌-tocopherol,linoleic acid,and琢-linolenic acid were also significantly increased in artificially fed Dabry’s loaches.This study provides a preliminary metabolic analysis of the nutritional value and food safety of P.dabryanus under different feeding methods,hoping to offer a theoretical basis for the development and utilization of Dabry’s loaches as a feed resource for endangered species such as crested ibises.
作者
邱国强
董南希
刘璐
李宗阳
石坚
俞越
刘小甜
李瑞
杨永春
宋厚辉
牛冬
QIU Guoqiang;DONG Nanxi;LIU Lu;LI Zongyang;SHI Jian;YU Yue;LIU Xiaotian;LI Rui;YANG Yongchun;SONG Houhui;NIU Dong(Deqing County Ecological Forestry Comprehensive Service Center,Deqing 313200,Zhejiang,China;College of Animal Science and Technology&College of Veterinary Medicine,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)
出处
《生命科学研究》
2025年第3期207-215,共9页
Life Science Research
基金
德清县生态林业综合服务中心项目(ZJXRDQ-2023-DT02)。
关键词
大鳞副泥鳅
饲喂方式
代谢组
营养价值
食物安全性
Paramisgurnus dabryanus
feeding method
metabolome
nutritional value
food safety
