摘要
为研究不同环境因素对紫贻贝(Mytilus galloprovincialis)幼贝足丝分泌的影响,选取山东省日照市养殖区的紫贻贝幼贝为研究对象,通过区组设计分别进行不同温度(17℃、22℃、27℃)和盐度(20、25、30),不同光照(10、100、10000 lx)和饥饿(0、24、48 h),不同干露(0、24、48 h)和氨氮(0、15、30 mg/L)条件的2因素交互作用实验。结果显示,温度和盐度均会对紫贻贝幼贝足丝分泌产生极显著影响(P<0.01),在实验温、盐条件下均呈显著弱负相关(R=-0.11/-0.16,P<0.05),其中温度27℃、盐度30%组别在不同时间点的紫贻贝幼贝足丝分泌能力显著低于其他组别(P<0.05)。光照对紫贻贝幼贝足丝分泌产生极显著影响(P<0.01),呈现极显著弱相关(R=0.14,P<0.01),而短期饥饿对紫贻贝幼贝足丝分泌影响不显著(P>0.05),低光照组(10 lx)紫贻贝幼贝足丝分泌能力显著高于其他组别。干露和氨氮均会对紫贻贝幼贝足丝分泌产生显著影响(P<0.05),其中干露与紫贻贝足丝分泌显著弱负相关(R=-0.09,P<0.05),而氨氮则显著负相关(R=-0.45,P<0.05),高氨氮(30 mg/L)和干露(48 h)组合紫贻贝幼贝足丝分泌能力显著低于其他组别(P<0.05)。研究结果可为紫贻贝的苗种转运、夹苗和健康养殖等生产实践提供有益参考。
To study the effects of environmental factors such as temperature,salinity,light,starvation,exposure to air,and ammonia-nitrogen on the productivity of byssus in Mytilus galloprovincialis,the juvenile blue mussels obtained from the cultivation rafts of Rizhao,Shandong Province were selected as research subjects.The two-factor interaction experiments between different temperatures(17℃,22℃,and 27℃),salinity concentrations(20‰,25‰,and 30‰),light intensities(10000,and 10000 lx),starvation times(0,24,48 h),air-exposure durations(0,24,48 h),and ammonia-nitrogen levels(0,15,and 30 mg/L)were conducted using block design.Both temperature and salinity significantly impacted productivity(P<0.01).Byssus production in the 27℃ and 30% salinity group was markedly lower than other groups at all time points(P<0.05).Within the experimental ranges,temperature and salinity showed a significant weak negative correlation(r=-0.11/-0.16,P<0.05)with byssus production.The light had a remarkable impact on byssus production(P<0.01),whereas short-term starvation had no significant effect(P>0.05).The dark group(10 lx)demonstrated a markedly elevated production of byssus than other groups;there was a weak correlation between light and byssus production(r=0.14,P<0.01).Air exposure and ammonia-nitrogen remarkably negatively influenced byssus production(P<0.05).High ammonia-nitrogen(30 mg/L)and prolonged air exposure(48 h)significantly constricted productivity(r=-0.09,P<0.05).These results can provide useful references for the practices of seedling transportation,clamping,and healthy cultivation of blue mussels.
作者
张守都
张伟
吴莹莹
甄秀维
邹杰
宋爱环
ZHANG Shoudu;ZHANG Wei;WU Yingying;ZHEN Xiuwei;ZOU Jie;SONG Aihuan(Marine Science Research Institute of Shandong Province(National Oceanographic Center,Qingdao),Qingdao 266104,China;Tianjin Agricultural University,Tianjin 300384,China;Guangxi Institute of Oceanology,Beihai 536000,China;Shandong Key Laboratory of Intelligent Marine Ranch(under preparation),Qingdao 266104,China)
出处
《海洋科学》
北大核心
2025年第2期56-66,共11页
Marine Sciences
基金
山东省农业良种工程项目(2024LZGCQY003,2021LZGC027,2022LZGC015)。
