摘要
热胁迫下珊瑚白化现象已被认为是全球珊瑚礁退化的主要驱动因素。热胁迫条件下珊瑚全共生功能体内的微生物群落组成、代谢和功能特性已有相关研究报道。然而,迄今还未见珊瑚健康-白化-恢复全过程中菌群结构及组成变化的相关研究。本研究以深圳海域澄黄滨珊瑚(Porites lutea)为研究对象,通过实验室模拟热胁迫下的珊瑚从健康-白化-恢复健康的过程,采用高通量测序技术和宏基因组测序技术分析珊瑚白化和恢复过程中的健康、开始白化、持续白化、开始恢复、恢复健康5个特征阶段珊瑚微生物群落的差异和功能基因变化。结果显示,随着温度的升高,变形杆菌门在白化过程中明显增多,在恢复过程中减少;拟杆菌门等在白化过程中减少,在恢复过程中增多。在珊瑚白化过程中,与压力耐受能力、生物膜形成、可移动因子及潜在致病性相关的细菌丰度显著增加,而群体感应相关细菌的丰度则在减少。4种微生物在珊瑚白化中发挥重要作用:不动杆菌属、罗尔斯通菌属和伯克霍尔德氏菌属是升温导致的珊瑚白化中的关键差异细菌类群;代尔夫特菌属可能通过群体感应机制调节其他菌群,以维持珊瑚微生物群落的稳定。本研究揭示了高温胁迫环境下珊瑚组织内微生物及其功能的变化,为阐明珊瑚白化过程中微生物-宿主互作机制提供分子依据。
Global coral bleaching under heat stress has been identified as a major driver of coral reef degradation.The composition,metabolism and functional characteristics of microbial communities in coral holosomes under heat stress has been reported.However,the changes in microbial structure and composition throughout the entire process of coral health-bleaching-recovery have not been studied so far.In this study,Porites lutea in Shenzhen Sea area was selected as the research object.The process of coral health-bleaching-recovery under heat stress was simulated in the laboratory.High-throughput sequencing and macro-genome sequencing technologies were used to analyse the differences in coral microbial communities and functional gene changes during five characteristic phases of coral bleaching and restoration:healthy,beginning of bleaching,continued bleaching,beginning of recover,and recovered.With the increase of temperature,Proteobacteria increased significantly during the bleaching process and decreased during recovery;Bacteroidota etc.decreased during bleaching and increased during recovery.During the coral bleaching process,the abundance of bacteria associated with stress tolerance,biofilm formation,mobile elements,and potential pathogenicity significantly increases.Conversely,the abundance of bacteria involved in quorum sensing decreases.Notably,four kinds of microbes play a crucial role in coral bleaching:Acinetobacter,Rhodobacter,and Burkholderia are key differential taxa in warming-induced coral bleaching,while Delftia may modulate other bacterial assemblages via quorum sensing mechanisms to maintain the stability of coral microbial communities.This study revealed the changes of microbes and their functions in coral tissues under high temperature stress,which provided molecular basis for elucidating the interaction mechanism between microbes and hosts during coral bleaching.
作者
刘子怡
刘永春
朱鸣
陈伯贵
郑惠娜
肖宝华
Liu Ziyi;Liu Yongchun;Zhu Ming;Chen Bogui;Zheng Huina;Xiao Baohua(College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;Shenzhen Research Institute,Guangdong Ocean University,Shenzhen 518108,China;Shenzhen Bihai Blue Sky Marine Technology Co.,LTD.,Shenzhen 518108,China;College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China)
出处
《海洋学报》
北大核心
2025年第5期48-63,共16页
基金
深圳市科技计划(JCYJ20200109144803833)
深圳市科技计划(可持续发展专项KCXFZ20211020165547011)
深圳市自然科学基金面上项目(JCYJ20230807120402005)。
关键词
珊瑚白化
微生物
高通量测序
宏基因组
coral bleaching
pure bacteria
high-throughput sequencing
metagenome
