摘要
滨海火电厂的循环水管道在停机检修时由于淤泥的厌氧发酵产生大量硫化氢(H_(2)S)和甲烷(CH_(4))等有毒有害气体,对设备和人员的安全造成严重危害,明确有毒有害气体的产生过程和机制,对开展相关的预防工作具有重要指导意义.通过构建淤泥-海水的厌氧发酵体系模拟循环水管道的环境,研究H_(2)S和CH_(4)的产生与微生物演替的关系.结果显示,模拟体系中H_(2)S在实验初期(0-15 d)迅速增加,而CH_(4)在25 d时达到峰值.在此过程中,微生物群落结构发生显著变化,在初始(0 d)时,Tindallia、Fusobacteriaceae和Bacteroidaceae为优势菌;在15-34 d,Lentimicrobiaceae、Thermovirgaceae、Gottschalkiaceae和Dethiosulfovibrionaceae的丰度逐渐上升并成为优势菌.H_(2)S含量的变化与Thermovirgaceae、Desulfobacteraceae等16个科的微生物呈正相关(r>0.8),CH_(4)含量的变化与Methanosarcinaceae、Sphingomonadaceae等46个科的微生物呈正相关(r>0.8),表明H_(2)S、CH_(4)的产生与Thermovirgaceae、Desulfobacteraceae等微生物相关.宏基因组数据分析显示H_(2)S主要由微生物通过同化硫酸盐还原、异化硫酸盐还原途径以及直接还原高半胱氨酸、甲硫醇和硫代硫酸盐产生;而CH_(4)主要通过CO_(2)还原、乙酸发酵和甲基化合物裂解途径产生.本研究揭示了滨海火电厂循环水管道中产生H_(2)S和CH_(4)等有毒有害气体的主要微生物组成,并初步明晰了其产生H_(2)S和CH_(4)的代谢途径,为滨海火电厂制定循环水管道中微生物产生H_(2)S等有毒有害气体的防治策略提供了理论依据.(图5表1参41)
The accumulation of toxic and harmful gases,such as H_(2)S and CH_(4),resulting from the anaerobic fermentation of sludge during maintenance in the circulating water pipelines of coastal thermal plants poses a serious threat to on-site workers and normal operation.Therefore,a clear understanding of the processes and mechanisms underlying the generation of these gases is crucial for performing preventive measures.In this study,an anaerobic jar system was established using sludge and seawater to simulate the anaerobic environment of a circulating water pipeline.The production of H_(2)S and CH_(4) along with their correlation with the microbial community structure were investigated.The H_(2)S levels increased rapidly within the first 15 d of fermentation,whereas the CH_(4) levels peaked at 25 d.Significant changes in microbial community structure were observed throughout this period.Tindallia,Fusobacteriaceae,and Bacteroidaceae dominated the early stages of fermentation,while the relative abundances of Lentimicrobiaceae,Thermovirgaceae,Gottschalkiaceae,and Dethiosulfovibrionaceae increased between 15 and 34 d.The production of H_(2)S was positively correlated with 16 families,including Thermovirgaceae and Desulfobacteraceae,whereas the production of CH_(4) was positively correlated with 46 families,including Methanosarcinaceae and Sphingomonadaceae.The core bacterial sulfate-reducing pathways involved in H_(2)S production include assimilatory and dissimilatory sulfate reduction and the reduction of homocysteine,methanethiol,and thiosulfate.Methane-producing archaea generate CH_(4) by reducing CO_(2),methanol,methylamines,and acetate.This study identified the microbial community structure that was correlated with the production of H_(2)S and CH_(4),preliminarily elucidated the biosynthetic pathways of these gases,and provided theoretical support for risk prevention and control of toxic and harmful gas production in the circulating water pipelines of coastal power plants.
作者
郑耿桦
尤亮
冯庭有
梁淑怡
谢建民
冯浩
王慧
蔡润林
ZHENG Genghua;YOU Liang;FENG Tingyou;LIANG Shuyi;XIE Jianming;FENG Hao;WANG Hui;CAI Ruilin(China Huaneng Shantou Haimen Power Generation Co.,Ltd.,Shantou 515132,China;Guangdong Provincial Key Laboratory of Marine Biology,College of Science,Shantou University,Shantou 515063,China)
出处
《应用与环境生物学报》
北大核心
2025年第5期836-846,共11页
Chinese Journal of Applied and Environmental Biology
基金
华能集团科技项目(HNKJ22-H68)资助。
关键词
循环水管道
硫化氢
甲烷
微生物群落组成
代谢途径
the circulating water pipeline
hydrogen sulfide
methane
microbial community structure
metabolic pathways
