摘要
目的探讨骐骥特殊膳食食品对小鼠运动性心肌损伤的保护作用。方法通过小鼠跑台实验构建小鼠运动性心肌损伤模型,同时灌胃给予骐骥特殊膳食食品,4周后进行跑台力竭实验,取血检测心肌钙蛋白T(cardiac troponin T,cTnT)、血清肌酸激酶(creatine kinase,CK)和乳酸脱氢酶(lactate dehydrogenase,LDH)。取心肌组织检测超氧化物歧化酶(superoxide dismutase,SOD)和丙二醛(malondialdehyde,MDA)含量。观察心肌病理学改变,透射电镜观察心肌超微结构变化。结果力竭训练4周后,与正常组小鼠相比,模型组小鼠血清cTnT、CK、心肌MDA水平显著升高(P<0.01),心肌SOD水平显著降低(P<0.01)。与模型组相比,高、中、低3个剂量组均可以显著降低力竭运动引起的血清cTnT和心肌MDA升高水平(P<0.01),且与正常组相比无显著性差异;3个剂量组均可以显著升高力竭运动引起的心肌SOD降低水平(P<0.05),与正常组无显著性差异。组织病理和超微病理检查发现,给药组心肌损伤明显减轻,中剂量组保护效果最优。结论骐骥特殊膳食食品对小鼠运动性心肌损伤具有明显的保护作用,其机制可能与抗氧化及减轻氧化应激有关。
Objective To investigate the protective effects of Qiji Special Dietary Food on exerciseinduced myocardial injury in mice.Methods An exercise-induced myocardial injury model was established using a treadmill running protocol,and at the same time,the modeled mice were administered Qiji Special Dietary Food via oral gavage,followed by a 4-week treadmill exhaustion test.Serum levels of cardiac troponin T(cTnT),creatine kinase(CK),and lactate dehydrogenase(LDH)were measured.Myocardial tissues were analyzed for superoxide dismutase(SOD)activity and malondialdehyde(MDA)content.Histopathological alterations and ultrastructural changes in myocardial tissue were evaluated using light microscopy and transmission electron microscopy(TEM).Results After 4 weeks of exhaustive training,compared to the control group,the model group exhibited significantly elevated serum cTnT,CK,and myocardial MDA levels(P<0.01),along with reduced myocardial SOD activity(P<0.01).Compared to the model group,high-,medium-,and low-dose treatment significantly attenuated the exhaustive exercise-induced increases in serum cTnT level and myocardial MDA content(P<0.01),restoring these indicators to the levels comparable to those of the normal control group(P<0.05).Additionally,all treatment groups had markedly increased myocardial SOD activity,with no significant difference from the normal group.Histopathological and ultrastructural analyses revealed markedly alleviated myocardial damage in the treatment groups,with the medium-dose group exhibiting the most pronounced protective effects.Conclusion Qiji Special Dietary Food demonstrates significant protective effects against exercise-induced myocardial injury in mice,which maybe associated with its antioxidant activity and mitigation of oxidative stress.
作者
冯旭
糜漫天
易龙
王伟
陈大贵
于旭东
陈松
FENG Xu;MI Mantian;YI Long;WANG Wei;CHEN Dagui;YU Xudong;CHEN Song(Naval Medical Center of PLA,Naval Medical University,Shanghai;Faculty of Military Preventive Medicine,Army Medical University(Third Military Medical University),Chongqing;Institute of Translational Medicine,Shanghai University,Shanghai,China)
出处
《陆军军医大学学报》
北大核心
2025年第13期1413-1419,共7页
Journal of Army Medical University
关键词
特殊膳食食品
运动
心肌损伤
special dietary food
exercise
myocardial injury
