摘要
玉滩湖是重庆市西部四大供水工程之一,有2条入河河流,是一个典型的多栖境生态系统。截至目前,尚未见玉滩湖鱼类组成和多样性的相关报道。本研究采用环境DNA(eDNA)技术,与传统鱼类网具调查结果进行对比分析,研究玉滩湖鱼类群落多样性和结构特征,探索eDNA技术在河流水库生境鱼类多样性中的应用潜力。结果显示:应用eDNA技术共检测出鱼类36种,隶属于4目11科32属,物种相对序列丰度分析发现鳙(Hypophthalmichthys nobilis)、鲢(Hypophthalmichthys molitrix)的相对丰度较高;传统鱼类网具调查共检测出21种鱼类,隶属于3目9科20属。eDNA技术与传统鱼类网具调查共检测出鱼类42种,其中15种为共有物种,占传统鱼类网具调查鱼类总数的71.43%。基于序列丰度的α和β多样性分析表明,湖区、河口和入库缓冲区鱼类组成和多样性呈现出一定的差异,河口和入库缓冲区的鱼类多样性高于湖区。综上,eDNA技术在鱼类物种检出率上高于传统鱼类网具调查,eDNA可作为一项重要的无损伤性鱼类资源监测辅助手段,在河流水库生境资源监测中进一步增加监测的可靠性。
Lake Yutan is one of the four main water supply initiatives in western Chongqing,with two inflow rivers.It is a typical multi-habitat ecosystem.To date,the fish composition and diversity in Lake Yutan have been scarcely reported.In this study,environmental DNA(eDNA)technology combined with the traditional fish survey was used to investigate the fish community and diversity in Lake Yutan,aiming to explore the potentials of eDNA technology in assessing fish diversity within river-reservoir habitats.The results showed that a total of 36 fish species categorized into 4 orders,11 families,and 32 genera were identified by eDNA technology.The analysis of relative sequence abundance revealed higher relative abundance for Hypophthalmichthys nobilis and Hypophthalmichthys molitrix.A total of 21 fish species,categorized into 3 orders,9 families,and 20 genera,were captured by the traditional fish survey.A total of 42 fish species were detected using the eDNA technology and traditional fish survey,with 15 species being jointly investigated,accounting for 71.43%of the total fishes captured through the traditional fish survey.The results ofαandβdiversity analysis based on sequence abundance revealed variations in fish composition and diversity across the lake region,estuary,and the buffer zone at the inlet.The fish diversity in the estuary and inlet buffer zone were higher than that observed in the lake region.In summary,our findings demonstrated that the application of eDNA technology had a superior detection rate for fish species compared with the traditional fish survey,and thereby could serve as an invaluable non-invasive supplementary tool for monitoring fishery resources and enhancing the reliability of river-reservoir habitat resource survey.
作者
沈子伟
谢浪
刘寒文
邓华堂
田辉伍
薛洋
陈大庆
段辛斌
Shen Ziwei;Xie Lang;Liu Hanwen;Deng Huatang;Tian Huiwu;Xue Yang;Chen Daqin;Duan Xinbin(National Agricultural Science Observing and Experimental Station of Chongqing,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,P.R.China;Chongqing Fisheries Technical Extension Center,Chongqing 400020,P.R.China)
出处
《湖泊科学》
北大核心
2025年第4期1096-1105,共10页
Journal of Lake Sciences
基金
国家重点研发计划项目(2022YFC3202001)
中华人民共和国农业农村部财政专项(西南地区重点水域渔业资源与环境调查)、中央级公益性科研院所基本科研业务费专项资金(YFI202414)
中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金(2023TD09)联合资助。
关键词
玉滩湖
EDNA
鱼类多样性
河流水库生境
Lake Yutan
environmental DNA
fish diversity
river-reservoir ecosystem
