摘要
目的通过观察十二时辰电针小鼠合谷穴的脑成像数据、脑血流动力学变化和脑功能连接差异,探讨增强合谷穴电针脑效应的最佳时间,以期为临床择时针灸取穴提供实验依据。方法将36只C57BL/6小鼠随机分为子时、丑时、寅时、卯时、辰时、巳时、午时、未时、申时、酉时、戌时、亥时12组,每组3只,实验时均给予相同模式的电针刺激。利用功能超声成像技术(Functional ultrasound imaging,FUS)以每0.4 s为一单位,采集每只小鼠电针前(静息态)、电针中(任务态)、电针后(任务后态)总计共420 s内的脑成像数据和脑血流动力学动态变化情况,并以海马区(Hippocampal region,HIP)为观察点,分析电针前后HIP和其他脑区功能连接的变化。结果与其他时辰相比,卯时组的全脑激活面积最大、平均激活信号强度最高,其海马区血流动力学信号增幅变化更为明显,且电针后血流信号强度仍明显高于电针前静息态;功能连接数据结果显示,以0.2作为标准值,相较于其他各组,卯时组电针前后发生改变的脑区数量最多,且只有卯时组小鼠双侧海马区之间的连接显著增强。结论不同时辰电针合谷穴的即时效应和脑功能连接存在明显差异,符合不同时辰的经脉气血流注盛衰规律,以卯时为最佳。
Objective To provide experimental evidence for selecting acupuncture timing in clinical practice,the optimal time for enhancing the brain effects of electroacupuncture at the Hegu acupoint(LI4)by observing brain imaging data,hemodynamic changes and differences in brain functional connectivity across the twelve traditional Chinese time periods were determined.Methods Thirty-six C57BL/6 mice were randomly divided into 12 groups corresponding to each of the twelve time periods(Zi,Chou,Yin,Mao,Chen,Si,Wu,Wei,Shen,You,Xu,Hai),with 3 mice per group.Each mouse received electroacupuncture stimulation using the same protocol.Brain imaging data and dynamic hemodynamic changes were collected using functional ultrasound imaging(FUS)ultrasound imaging technology every 0.4 s over a total duration of 420 s,covering pre-acupuncture(resting state),during acupuncture(task state),and post-acupuncture(post-task state)phases.The hippocampal region(HIP)was used as the observation point to analyze changes in functional connectivity between HIP and other brain regions before and after acupuncture.Results Compared to other time periods,the Mao group exhibited the largest whole-brain activation area and the highest average activation signal intensity.The hemodynamic signal increase in the hippocampal region was more pronounced,and the post-acupuncture blood flow signal intensity remained significantly higher than the pre-acupuncture resting state.Functional connectivity data revealed that,using 0.2 as the standard value,the Mao group showed the greatest number of altered brain regions before and after acupuncture.Notably,only in the Mao group was there a significant enhancement in connectivity between the bilateral hippocampal regions.Conclusion The immediate effects of electroacupuncture at the Hegu acupoint(LI4)and brain functional connectivity vary significantly across different time periods,aligning with the traditional Chinese medicine theory of meridian qi and blood flow.Mao time is identified as the optimal period.
作者
许淑鱼
张帅
刘欣媛
李蔚娴
孙国杰
田青
杜艳军
XU Shuyu;ZHANG Shuai;LIU Xinyuan;LI Weixian;SUN Guojie;TIAN Qing;DU Yanjun(College of Acupuncture-Moxibustion and Orthopedics,Hubei University of Chinese Medicine,Wuhan 430061,China;Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;Hubei Shizhen Laboratory,Hubei International Science and Technology Cooperation Base of Preventive Treatment by Acupuncture and Moxibustion,Wuhan 430060,China)
出处
《世界科学技术-中医药现代化》
北大核心
2025年第5期1244-1252,共9页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委员会面上项目(82375464):电针调节IL-3/IL-3Rα介导AC-MG交互以促小胶质细胞吞噬Aβ机制研究,负责人:杜艳军
国家自然科学基金面上项目(82074566):基于p300/CBP/CREB通路研究电针调控阿尔茨海默病小胶质细胞M2/M1极化表型平衡的组蛋白乙酰化机制,负责人:杜艳军
湖北省联合基金重点项目(2023AFD112):“益肾调督”法电针调控AC-MG交互串扰以促AD脑内稳态平衡的机制研究,负责人:杜艳军。
关键词
时间针灸
气血流注
子午流注
合谷
海马
脑血流动力学
脑功能连接
Chrono-acupuncture
Qi and blood flow
Zi Wu Liu Zhu
Hegu acupoint(LI4)
Hippocampus
Cerebral hemodynamics
Brain functional connectivity
