摘要
目的课题组在前期工作中制备了骨碎补总黄酮主动骨靶向递药系统(Aln-gel-BMSN-TFRD),本研究旨在探索Aln-gel-BMSN-TFRD对MC3T3-E1细胞增殖凋亡和成骨分化的作用及机制,为临床治疗骨质疏松症(osteoporosis,OP)提供更多方法。方法通过CCK-8实验检测Aln-gel-BMSN-TFRD对细胞活性的影响并筛选最佳浓度,流式细胞术检测其对细胞凋亡的影响,茜素红染色和碱性磷酸酶染色观察其对细胞成骨分化的影响,qPCR和Western blot检测其对细胞Runx2、Osx、BMP2、Wnt1、Wnt3a和β-catenin表达的影响。结果相较于TFRD,Aln-gel-BMSN-TFRD能更好地促进细胞增殖、抑制细胞凋亡、促进成骨分化、上调Runx2、Osx、BMP2、Wnt1、Wnt3a和β-catenin的表达。结论Aln-gel-BMSN-TFRD在保护细胞、促进成骨分化、上调成骨相关因子表达、激活Wnt/β-catenin通路方面的作用优于TFRD,有作为OP新型治疗方法的潜力。
Objective In previous work,our research team developed a bone-targeting drug delivery system for the total flavonoids of rhizoma drynariae(Aln-gel-BMSN-TFRD).This study aimed to explore the effects and mechanisms of Aln-gel-BMSN-TFRD on the proliferation,apoptosis,and osteogenic differentiation of MC3T3-E1 cells,providing potential new method for the clinical treatment of osteoporosis(OP).Methods The impact of Aln-gel-BMSN-TFRD on cell viability was assessed using the CCK-8 assay to determine the optimal concentration.Flow cytometry was used to evaluate its effect on cell apoptosis.Alizarin Red and alkaline phosphatase staining were employed to observe its influence on osteogenic differentiation.The expression levels of Runx2,Osx,BMP2,Wnt1,Wnt3a,andβ-catenin were measured using qPCR and Western blot.Results Compared to TFRD,Aln-gel-BMSN-TFRD significantly enhanced cell proliferation,inhibited apoptosis,promoted osteogenic differentiation,and upregulated the expression of Runx2,Osx,BMP2,Wnt1,Wnt3a,andβ-catenin.Conclusion Aln-gel-BMSN-TFRD is superior to TFRD in protecting cells,promoting osteogenic differentiation,upregulating the expression of osteogenic related factors,and activating Wnt/β-catenin pathway.It has the potential to be used as a new treatment for OP.
作者
金天翔
胡继红
毛勇
孔泳
高俊
JIN Tianxiang;HU Jihong;MAO Yong;KONG Yong;GAO Jun(Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine,Wenzhou 325000,China;The Third Affiliated Hospital of Nanjing Medical University,Changzhou 213002,China;Guangzhou Panyu District Hospital of Traditional Chinese Medicine,Guangzhou 511400,China;School of Petrochemical Engineering of Changzhou University,Changzhou 213164,China;Changzhou Hospital of Traditional Chinese Medicine,Changzhou 213002,China)
出处
《中国骨质疏松杂志》
北大核心
2025年第6期789-798,共10页
Chinese Journal of Osteoporosis
基金
国家自然科学基金(82374479)
江苏省自然科学基金(BK20211066)
江苏省中医管理局科技项目(MS2022079)
广州市科技局科技计划项目(202201011670)。
关键词
骨质疏松症
骨碎补总黄酮
骨靶向
递药系统
成骨分化
osteoporosis
total flavonoids of rhizoma drynariae
bone targeting
delivery system
osteogenic differentiation
