摘要
本研究基于网络药理学和分子对接技术,旨在探讨绿原酸缓解绵羊羔羊肠道腹泻的作用机制。通过TCMSP、Pharm Mapper和Swiss Target Prediction数据库预测绿原酸的潜在作用靶点,并结合Gene Card和OMIM数据库获取绵羊羔羊腹泻的潜在靶点。构建蛋白质-蛋白质相互作用网络并筛选出核心靶点。并通过DAVID数据库进行GO功能和KEGG通路富集分析,揭示绿原酸调控的生物过程和信号通路,之后采用分子对接技术检验对接稳定性。结果表明:绿原酸缓解绵羊羔羊肠道腹泻的潜在靶点有36个,其中核心靶点有7个,分别是半胱天冬酶3、连环蛋白β1、表皮生长因子受体、前类淀粉蛋白质、原癌基因酪氨酸蛋白激酶、G1/S-特定周期蛋白D1和肝细胞生长因子受体。GO功能和KEGG通路富集分析显示,靶点作用包括25种生物过程、11种细胞组分和8种分子功能,富集到黏着斑、黏附连接、丝裂原活化蛋白激酶、磷脂酰肌醇3激酶-蛋白激酶B等39条通路。分子对接结果显示,绿原酸与核心靶点的结合能均小于-25.12 kJ/mol。综上所述,本研究筛选了绿原酸缓解绵羊羔羊腹泻的作用靶点和相关通路,为绿原酸作为绿色饲料添加剂在绵羊羔羊养殖中的应用提供了理论依据。
Based on network pharmacology and molecular docking technology,this study aimed to explore the target and pathway regulation mechanism of chlorogenic acid in alleviating intestinal diarrhea in sheep lambs.The potential targets of chlorogenic acid were predicted by TCMSP,Pharm Mapper and Swiss Target Prediction database,and the potential targets of sheep lamb diarrhea were obtained by combining Gene Card and OMIM database.The protein-protein interaction network was constructed and the core target was screened.Furthermore,GO function and KEGG pathway enrichment analysis were conducted through the DAVID database to reveal the biological processes and signaling pathways regulated by chlorogenic acid,and then molecular docking technology was used to test docking activity.The results showed that there were 36 potential targets for chlorogenic acid to alleviate intestinal diarrhea in sheep lambs among which 7 core targets were caspase 3,catenin beta 1,epidermal growth factor receptor,amyloid-beta precursor protein,proto-oncogene tyrosine-protein kinase,G1/S-specific cyclin D1 and hepatocyte growth factor receptor.The enrichment analysis of GO function and KEGG pathway showed that the target effects included 25 biological processes,11 cell components and 8 molecular functions(P<0.05),and 39 pathways such as adhesive plaques,adhesive junctions,mitogen-activated protein kinase and phosphatidylinositol 3-kinase-protein kinase B were enriched.Molecular docking results showed that the binding energy between chlorogenic acid and core target was less than-25.12 kJ/mol.In summary,this study screens the targets and related pathways of chlorogenic acid regulation of diarrhea in sheep lambs,providing a theoretical basis for the application of chlorogenic acid as a green feed additive in lamb breeding.
作者
陈鹏
义拉勒特
萨初拉
张春华
王博
金鹿
李胜利
杨鼎
孙海洲
CHEN Peng;Yilalete;SA Chula;ZHANG Chunhua;WANG Bo;JIN Lu;LI Shengli;YANG Ding;SUN Haizhou(College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China;Inner Mongolia Key Laboratory of Herbivores Nutrition,Key Laboratory of Grass-Feeding Livestock Healthy Breeding and Livestock Product Quality Control(Co-Construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)
出处
《动物营养学报》
北大核心
2025年第6期4090-4104,共15页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
内蒙古自治区科技重大专项项目(2021ZD0012)
国家绒毛用羊产业技术体系(CARS-39-11)
国家重点研发计划项目(2022YFD1301102)。
关键词
绿原酸
绵羊羔羊
肠道腹泻
网络药理学
分子对接
chlorogenic acid
sheep lamb
intestinal diarrhea
network pharmacology
molecular docking
