摘要
为了探究金耳子实体胶质(Naematelia aurantialba fruit body glial,简称NAG)对小鼠血清细胞因子及肠道菌群的调节作用,分析NAG的成分和特征,并将其对正常小鼠进行为期21 d的灌胃处理,收集血清样本和粪便样本等,采用酶联免疫吸附法(ELISA)测定血清细胞因子含量,气-质联用(GC-MS)技术测定盲肠内容物短链脂肪酸(SCFAs)含量,16S rRNA测序技术分析小鼠粪便菌群并进行基因功能预测。结果显示:NAG主要营养成分为膳食纤维,其摄入使小鼠血清细胞因子TNF-α含量增加了1.21倍,IL-1β含量降低100%,IL-10含量增加了4.42倍;盲肠内容物中短链脂肪酸丁酸的含量显著增加(P<0.05);粪便中毛螺菌科(Lachnospiraceae)、瘤胃球菌科(Ruminococcaceae)相对丰度显著增加(P<0.05);肠道菌群基因功能中分泌系统、硫中继系统和谷氨酸能突触相对丰度显著增加(P<0.05),传染疾病、其他聚糖降解和脂多糖生物合成蛋白相对丰度显著降低(P<0.05)。表明NAG能够通过促进肠道益生菌群的增殖和短链脂肪酸丁酸的分泌,调节肠道菌群功能,使肠道微生态趋于健康,有助于提升免疫力、改善代谢和降低慢性病风险。
To investigate the regulatory effects of Naematelia aurantialba fruitbody glial(NAG)on the serum cytokines and intestinal flora of mice,the composition and characteristics of NAG were first analyzed.Normal mice were then administered NAG via gavage for 21 days.Serum and fecal samples were collected,and enzyme-linked immunosorbent assay(ELISA)was utilized to quantify the serum cytokine levels.Gas chromatography-mass spectrometry(GC-MS)was employed to measure the short-chain fatty acids(SCFAs)in the cecal contents.Additionally,16S rRNA sequencing technology was applied to analyze the mouse fecal microbiota,and functional predictions were performed based on genetic analysis.The results showed that the main nutritional component of NAG was dietary fiber.NAG significantly increased the serum TNF-αlevel 1.21 times,reduced the IL-1βcontent by 100%,and elevated the IL-10 level 4.42 times.Furthermore,the concentration of butyric acid in the cecal contents was markedly increased(P<0.05),and the relative abundances of Lachnospiraceae and Ruminococcaceae in the feces were also increased significantly(P<0.05).Functional analysis revealed that the secretion system,sulfur relay system,and glutamatergic synapse were relatively more abundant in the intestinal microbiota,with significant increases in their abundance(P<0.05).Conversely,the relative abundances of genes associated with infectious diseases,other glycan degradation,and lipopolysaccharide biosynthesis proteins were decreased(P<0.05).These findings indicate that NAG can regulate the intestinal microbiota function by promoting the proliferation of beneficial gut microbiota and the secretion of SCFAs(e.g.,butyrate),thereby promoting a shift toward a healthier intestinal microecology.This modulation is conducive to enhancing immunity,improving metabolism,and reducing the risk of chronic diseases.
作者
李文芝
马云蕊
罗祥英
周云燕
李荣春
LI Wenzhi;MA Yunrui;LUO Xiangying;ZHOU Yunyan;LI Rongchun(Yunnan Junshijie Biotech Ltd.,Kunming 651708,China;Yunnan(Rare Edible Fungi)Enterprise Technology Center,Kunming 651708,China;Kunming(Edible Fungi)Enterprise Technology Center,Kunming 651708,China)
出处
《食药用菌》
2025年第3期186-194,共9页
Edible and Medicinal Mushrooms
基金
云南(昆明)张劲松药用真菌专家工作站(No.YSZJGZZ-2022043)
云南省张劲松专家工作站(No.202305AF150084)。
关键词
金耳
NAG
膳食纤维
短链脂肪酸
肠道微生物
Naematelia aurantialba
NAG
dietary fiber
short-chain fatty acids
intestinal microbial
