摘要
目的 基于“胃不和则卧不安”理论,采用两样本孟德尔随机化方法探讨胃食管反流病与失眠间的因果关系。方法 汇总数据来源于全基因组关联研究,暴露因素为胃食管反流病,结局变量为失眠;孟德尔随机化分析通过逆方差加权法、MR-Egger法、简单模型法、加权模型法和加权中位数法实现;敏感性分析包括多效性检验、异质性检验和逐个剔除检验,且依次采用MR-Egger法、Cochran Q检验法和留一法计算。结果 共筛选75个单核苷酸多态性。逆方差加权法结果显示,胃食管反流病会增加失眠发生风险(OR=1.255,95%CI:1.071-1.470,P<0.05);加权中位数法同样证实胃食管反流病与失眠具有正向因果关系(OR=1.403,95%CI:1.117-1.762,P<0.05)。多项敏感性分析提示研究孟德尔随机化分析结果不存在多效性和异质性,验证了研究结果的稳定性。结论 胃食管反流病与失眠之间存在正向的因果关系,从遗传角度丰富了“胃不和则卧不安”理论内涵,亦为进一步深入研究奠定了基础。
Objective Based on the theory of"stomach disharmony leads to restlessness",the causal relationship between gastroesophageal reflux disease and insomnia was discussed by Mendel randomization method with two samples.Methods The data were collected from genome-wide association studies,the exposure factor was gastroesophageal reflux disease,and the outcome variable was insomnia.Mendel randomization analysis is realized by inverse variance weighted method,MR-Egger method,simple model method,weighted model method and weighted median method.Sensitivity analysis includes pleiotropic test,heterogeneity test and one-by-one elimination test,and is calculated by MR-Egger method,Cochran Q test and one-out-of-one method in turn.Results A total of 75 single nucleotide polymorphisms(SNPs)were screened.The inverse variance weighted method showed that gastroesophageal reflux disease increased the risk of insomnia(OR=1.255,95%CI:1.071-1.470,P<0.05).The weighted median method also confirmed that there was a positive causal relationship between gastroesophageal reflux disease and insomnia(OR=1.403,95%CI:1.117-1.762,P<0.05).Multiple sensitivity analysis indicated that there was no pleiotropy and heterogeneity in the results of Mendelian randomized analysis,which verified the stability of the research results.Conclusion There is a positive causal relationship between gastroesophageal reflux disease and insomnia,which enriches the theoretical connotation of"stomach disharmony leads to restlessness"from the genetic point of view and lays a foundation for further study.
作者
吴千言
徐小港
张清源
张敬文
章德林
WU Qianyan;XU Xiaogang;ZHANG Qingyuan;ZHANG Jingwen;ZHANG Delin(College of Traditional Chinese Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,China)
出处
《世界科学技术-中医药现代化》
北大核心
2025年第3期867-877,共11页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
江西中医药大学首批校级科技创新团队(CXTD22016):中医体质-状态辨识健康管理研究团队,负责人:章德林
江西省研究生创新专项资金项目(YC2024-B227):基于“胃不和则卧不安”理论从全基因组关联性探讨胃食管反流病与失眠的因果关系,负责人:吴千言。
关键词
胃不和则卧不安
胃食管反流病
失眠
关联性
孟德尔随机化
Disharmony of stomach leads to restlessness
Gastroesophageal reflux disease
Insomnia
Relevance
Mendel randomization
