摘要
目的分析2017—2022年成都市新都区7起食源性暴发事件分离的沙门菌的生物学特征和耐药特征,为新都区制定沙门菌食源性疾病暴发防控策略,指导临床合理用药提供依据。方法对2017—2022年成都市新都区的7起暴发事件(3起肠炎,4起鼠伤寒)分离的39株沙门菌,经生化和血清学鉴定,用微量肉汤稀释法进行15种抗菌药物的药敏试验,使用XbaI限制性内切酶处理后进行PFGE实验,用BioNumerics软件进行聚类分析,使用SPSS 19.0软件进行统计学分析,组间计数资料的比较用χ^(2)检验,P<0.05为差异有统计学意义。结果7起暴发事件的39株沙门菌耐药严重,对萘啶酸耐药率高达97.44%,氨苄西林耐药率为74.36%,多粘菌素E耐药率为71.79%,四环素、复方新诺明、氨苄西林/舒巴坦和头孢唑林耐药率在10.26%~25.64%,对其他抗生素均不耐药。多重耐药率为74.36%(29/39),肠炎沙门菌多重耐药率92.59%(25/27)显著高于鼠伤寒沙门菌33.33%(4/12)。4起事件12株鼠伤寒沙门菌分4个带型,每起事件内分离株带型100%相同,不同事件带型相似度在88.20%~100.00%之间。3起事件27株肠炎沙门菌分3个带型,相似度在90.00%~100.00%之间,其中两起事件带型100%相同。结论新都区沙门菌引起的食源性暴发事件主要为鼠伤寒和肠炎沙门菌;肠炎沙门菌耐药严重;PFGE聚类分析显示,可能存在沙门菌持续流行的情况。
Objective To analyze the biological and drug resistance characteristics of Salmonella isolated from 7 foodborne outbreaks in Xindu District,Chengdu City from 2017 to 2022,and to provide basis for formulating the prevention and control strategy of Salmonella foodborne outbreaks in Xindu District and guiding rational clinical medication.Methods The 39 strains of Salmonella isolated from 7 outbreaks(3 enteritis and 4 typhimurium)in Xindu District of Chengdu City from 2017 to 2022 were identified by biochemical and serological methods,and the drug susceptibility test of 15 antibacterial agents was performed by microbroth dilution method.The PFGE(pulsed field gel electrophoresis)test was performed after treatment with Xbal restriction enzyme.BioNumerics software was used for cluster analysis,SPSS 19.0 software was used for statistical analysis,and statistical data between groups were compared with x2 test.P<0.05 was considered statistically significant.Results The 39 Salmonella strains isolated from 7 outbreaks were severely resistant,with a resistance rate of 97.44%to nalidixic acid,74.36%to ampicillin,and 71.79%to polymyxin E.The resistance rates to tetracycline,compound sulfamethoxazole,ampicillin/sulbactam and cefazolin ranged from 10.26%to 25.64%,and they were not resistant to other antibiotics.The multidrug resistance rate was 74.36%(29/39),and the multidrug resistance rate of S.Enteritidis was 92.59%(25/27),which was significantly higher than that of S.Typhimurium(33.33%)(4/12).The 12 strains of S.Typhimurium from 4 events were divided into 4 bands,with 100.00%identical bands in each event.The similarity of different event bands ranged from 88.2%to 100.00%.The 27 strains of S.Enteritidis were divided into 3 bands in 3 events,with a similarity between 90.00%and 100%.Among them,the bands in two events were 100%identical.Conclusions The Salmonella foodbome outbreaks in Xindu District were mainly caused by S.Typhimurium and S.Enteritidis.The antibiotic resistance of S.Enteritidis was severe.PFGE cluster analysis showed that there may be a sustained epidemic situation of Salmonella.
作者
王尔群
宋志琴
秦超
黄伟峰
WANG Erqun;SONG Zhiqin;QIN Chao;HUANG Weifeng(Chengdu Xindu Center for Disease Control and Prevention,Chengdu 610500,Sichuan Province,China;Sichuan Center for Disease Control and Prevention,Chengdu 610041,Sichuan Province,China)
出处
《预防医学情报杂志》
2025年第5期681-686,共6页
Journal of Preventive Medicine Information
关键词
沙门菌
暴发
药敏试验
抗生素
脉冲凝胶电泳
Salmonella
outbreak
drug sensitivity test
antibiotics
pulsed gel electrophoresis
