摘要
目的:探讨Pd/ZIF-8纳米酶介导的光热与酶促协同抗菌性能,评估其在生物医学领域的应用潜力。方法:通过水热法制备Pd/ZIF-8纳米酶,并通过透射电子显微镜(TEM)和X射线衍射(XRD)进行结构表征。评估Pd/ZIF-8纳米酶的光热转换性能以及其模拟类过氧化物酶(POD)和谷胱甘肽过氧化物酶(GPx)活性的能力。采用细胞计数试剂盒(CCK-8)检测Pd/ZIF-8对人皮肤成纤维细胞(HDF-a)的毒性情况,同时利用红细胞溶血试验检测其血液相容性。检测在808 nm近红外光照射条件下Pd/ZIF-8对革兰阴性菌(大肠杆菌,E.coli)和革兰阳性菌(耐甲氧西林金黄色葡萄球菌,MRSA)的抗菌性能。结果:TEM和XRD分析证实Pd/ZIF-8纳米酶合成成功。Pd/ZIF-8纳米酶具有良好的光热性能,光热转换率达65.3%,且稳定性良好。同时,Pd/ZIF-8纳米酶表现出优异的双重类酶活性(POD和GPx)。细胞毒性及溶血结果表明Pd/ZIF-8具有良好的生物相容性。在近红外光照射下,Pd/ZIF-8对E.coli和MRSA的抗菌率分别为97.3%和99.41%(P<0.0001)。结论:Pd/ZIF-8纳米酶具有介导光热与酶促达到协同抗菌的性能,该纳米材料在抗感染治疗领域展现出良好的应用前景,为开发新型抗菌剂提供了重要思路。
Objective:To explore the synergistic antibacterial performance of photothermal and enzymatic catalysis mediated by Pd/ZIF-8 nanozyme,and assess its potential for biomedical applications.Methods:The Pd/ZIF-8 nanozyme was prepared by the hydrothermal method,and its structure was characterized by transmission electron microscopy(TEM)and X-ray diffraction(XRD).The photothermal conversion performance of the Pd/ZIF-8 nanozyme and its ability to mimic the activities of peroxidase(POD)and glutathione peroxidase(GPx)were evaluated.The cell counting kit-8(CCK-8)method was used to detect the toxicity of Pd/ZIF-8 to human dermal fibroblasts(HDF-a),and the erythrocyte hemolysis test was used to detect its blood compatibility.The antibacterial performance of Pd/ZIF-8 against Gram-negative bacteria(Escherichia coli,E.coli)and Gram-positive bacteria(methicillin-resistant Staphylococcus aureus,MRSA)under the irradiation of 808 nm near-infrared light was detected.Results:TEM and XRD analyses confirmed the successful synthesis of the Pd/ZIF-8 nanozyme.The Pd/ZIF-8 nanozyme exhibited good photothermal performance with a photothermal conversion rate of 65.3%and good stability.Meanwhile,the Pd/ZIF-8 nanozyme exhibited excellent dual enzyme-like activities(POD and GPx).The results of cytotoxicity and hemolysis tests indicated that Pd/ZIF-8 had good biocompatibility.Under near infrared light irradiation,the antibacterial rates of Pd/ZIF-8 against E.coli and MRSA were 97.3%and 99.41%,respectively(P<0.0001).Conclusion:The Pd/ZIF-8 nanozyme has the ability to mediate photothermal and enzymatic promotion to achieve synergistic antimicrobial properties.This nanomaterial shows promising application prospects in the field of anti-infection treatment and provides important ideas for the development of new antibacterial agents.
作者
莫苗
黄章睿
赵昕
钟静萍
MO Miao;HUANG Zhangrui;ZHAO Xin;ZHONG Jingping(Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed by the Province and Ministry,Guangxi Medical University,Nanning 530021,China;Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration,Guangxi Medical University,Nanning 530021,China)
出处
《广西医科大学学报》
2025年第3期395-401,共7页
Journal of Guangxi Medical University
基金
国家自然科学基金资助项目(No.52301303)。
关键词
纳米酶
近红外光
抗菌活性
酶催化
nanozyme
near-infrared light
antibacterial activity
enzymatic catalysis
