摘要
目的:探究不同运动方式对2型糖尿病动脉粥样硬化小鼠体重、血糖和动脉粥样硬化斑块的作用,进一步比较各运动方式对其内皮祖细胞(EPCs)功能的影响。方法:取高脂喂养联合链脲佐菌素(STZ)诱导的ApoE-/-小鼠构建成2型糖尿病动脉粥样硬化模型,将32只小鼠随机分到空白对照组(control组)、有氧运动(AE)组、抗阻运动(RE)组、有氧联合抗阻运动(AE+RE)组。于运动干预0周、2周、4周、6周、8周检测小鼠体重和空腹血糖变化。干预8周后分离小鼠主动脉进行大体油红O染色和切片苏木精—伊红(HE)染色,并提取各组小鼠骨髓EPCs细胞进行增殖、迁移、黏附和血管生成功能检测。结果:实施运动干预8周后,AE组和AE+RE组的体重较control组下降(P<0.05)。各运动组血糖均显著下降,差异具有统计学意义(P<0.05)。主动脉大体油红O染色显示,各运动组的动脉粥样硬化斑块与血管总面积比值均较control组显著下降(P<0.05);主动脉切片HE染色镜下结果显示,与control组相比,AE组和RE组镜下斑块与血管内径比值无显著差异(P>0.05),而AE+RE组变小(P<0.05)。各运动组EPCs增殖、迁移和血管生成均较control组有显著改善(P<0.05);黏附能力AE组和RE组较control组无显著差异(P>0.05),AE+RE组强于control组(P<0.05)。结论:相较单一的AE或RE方式,AE联合RE抗阻运动对2型糖尿病动脉粥样硬化小鼠体重、血糖及动脉粥样硬化的改善作用更佳,并且对促进其EPCs功能也具有显著的积极影响。
Objective:To investigate the effects of different exercise modalities on body weight,blood glucose,and atherosclerotic plaques in mice with type 2 diabetes mellitus(T2DM)and atherosclerosis,and further compare the impacts of these exercise modalities on the function of endothelial progenitor cells(EPCs).Methods:ApoE-/-mice induced by high-fat feeding combined with streptozotocin(STZ)were constructed into T2DM atherosclerosis model,and 32 mice were randomly divided into blank control group(control group),aerobic exercise(AE)group,resistance exercise(RE)group and aerobic combined with resistance exercise(AE+RE)group.Mice were assessed for the changes of body weight and fasting blood glucose at 0,2,4,6,and 8 weeks of exercise intervention.After 8 weeks,the mouse aortas were isolated for Oil Red O staining and hematoxylin-eosin(HE)staining,and the mouse bone marrow EPCs were extracted from each group for proliferation,migration,adhesion,and angiogenesis assessments.Results:After 8 weeks of exercise intervention,there were significant differences in the weight loss between the AE group and the AE+RE group compared with the control group(P<0.05).Blood glucose levels were significantly decreased in all exercise groups(P<0.05).Oil Red O staining of the aorta showed a significant reduction in the ratio of atherosclerotic plaques to the total vessel area in all exercise groups compared with the control group(P<0.05).HE staining of aortic sections results indicated no significant difference in plaque-to-vessel diameter ratio between the AE and RE groups compared with the control group(P>0.05),while the ratio in the AE+RE group was smaller(P<0.05).EPCs in all exercise groups showed significant improvements in proliferation,migration and angiogenesis compared with the control group(P<0.05).Adhesion ability was not significantly different between AE and RE groups compared with the control group(P>0.05),while the AE+RE group showed significantly better adhesion(P<0.05),and the difference was statistically significant.Conclusion:Compared with single AE or RE methods,AE combined with RE exercise has a better improvement effect on body weight,blood glucose and atherosclerosis in T2DM atherosclerosis mice.It also has a significant positive effect on the promotion of EPCs function.
作者
黄峥
徐子岚
李苏蓝
吴妮晓
戴霞
HUANG Zheng;XU Zilan;LI Sulan;WU Nixiao;DAI Xia(The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)
出处
《广西医科大学学报》
2025年第3期360-367,共8页
Journal of Guangxi Medical University
基金
国家自然科学基金资助项目(No.82160440)
广西自然科学基金资助项目(No.2023GXNSFAA026323)
广西研究生教育创新计划资助项目(No.YCSW2024246)。
关键词
2型糖尿病
动脉粥样硬化
运动
内皮祖细胞
type 2 diabetes mellitus
atherosclerosis
exercise
endothelial progenitor cells
