摘要
【目的】Transformer 2(Tra2)基因作为多种昆虫的性别决定关键基因,与Tra协同调控下游Dsx基因进行性特异性剪接,从而控制雌雄分化。本研究旨在鉴定意大利蝗Calliptamus italicus的Tra2基因结构特征及其在不同发育时期的表达模式。【方法】基于意大利蝗卵的转录组数据库以及同源性比对结果,克隆了Tra2的开放阅读框(Open reading frame,ORF)序列片段,并进行结构预测、多重序列比对以及进化树分析;采用qRT-PCR技术检测意大利蝗卵期、不同发育龄期及成虫精巢和卵巢的Tra2表达量。【结果】意大利蝗Tra2基因ORF序列含672 bp,编码223个氨基酸,Tra2编码的蛋白具有RRM-TRA结构域及特有Linker区。系统进化关系表明,意大利蝗Tra2编码的蛋白与鳞翅目亲缘关系最接近。Tra2在意大利蝗不同发育时期均有表达,在蝗卵发育过程中,阶段Ⅰ与阶段Ⅷ表达量较高;蝗蝻与成虫的Tra2表达量均为雌性显著高于雄性(P<0.05)。【结论】明确了意大利蝗的Tra2基因结构特征,且雌虫Tra2的表达水平显著高于雄性,说明该基因对雌虫性器官的发育影响大于雄虫,为深入阐明Tra2在意大利蝗性别决定过程的调控作用奠定了基础。
[Aim]To identify structural characteristics of the Transformer 2(Tra2)gene,a key gene involved in sex determination in many insects,and quantify its expression in different developmental stages of Calliptamus italicus.[Methods]Based on the transcriptome database of C.italicus eggs and a comparison of homologous genes in other species,the open reading frame(ORF)sequence fragment of Tra2 was cloned.The predicted structure,multiple sequence alignment and a phylogenetic tree of the cloned sequence were obtained using bioinformatic software.qRT-PCR was used to detect Tra2 expression in eggs,nymphs,and in the testes and ovaries of adult C.italicus.[Results]The ORF sequence of the Tra2 gene contains 672 bp and encodes 223 amino acids.The protein encoded by Tra2 has an RRM-TRA domain and a unique linker region.A phylogenetic tree indicates that the protein encoded by Tra2 in C.italicus is most closely related to homologous lepidopteran proteins.Tra2 was expressed in different developmental stages of C.italicus but expression was the highest during stageІand of eggⅧdevelopment.Tra2 expression was significantly higher in females than in males in both nymphs and adults(P<0.05).[Conclusion]Tra2 in C.italicus is closely related to homologous genes in other lepidopteran species.Expression in females is significantly higher than in males,suggesting that this gene has greater influence on the development of female than male sexual organs.These results lay a foundation for further elucidating the role of Tra2 in sex determination in C.italicus.
作者
张韦萱
蒋思涵
扈鸿霞
赵娜
季荣
叶小芳
ZHANG Wei-Xuan;JIANG Si-Han;HU Hong-Xia;ZHAO Na;JI Rong;YE Xiao-Fang(College of Life Science,Xinjiang Normal University,International Center for the Collaborative Management of Cross-border Pest in Central Asia,Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology,Key Laboratory of Special Environment Biodiversity Application and Regulation in Xinjiang,Urumqi 830054,China)
出处
《应用昆虫学报》
北大核心
2025年第2期381-389,共9页
Chinese Journal of Applied Entomology
基金
新疆维吾尔自治区天山创新团队计划(2024D14006)
新疆维吾尔自治区天山英才青年拔尖人才项目(20243123638)
新疆师范大学青年拔尖人才项目(XJNUQB2022-30)。
关键词
意大利蝗
Tra2
结构特征
表达模式
性别决定
Calliptamus italicus
Tra2
structure characteristics
expression pattern
sex determination
