摘要
低温胁迫下有机体可通过改变膜系统脂肪酸组成与比例,以调节其流动性应答与适应冷环境,亚麻酸及其代谢中间产物是油菜脂肪酸代谢的重要组成部分,本研究以两个抗寒性不同的甘蓝型冬油菜品种NS(强抗寒)和NF(低温敏感)为试验材料,通过转录组学分析,探讨甘蓝型冬油菜亚麻酸代谢参与调控低温应答的分子机制。结果表明,亚麻酸代谢通路在常温和-4℃处理12 h时均显著富集,在两个品种间筛选得30个仅在低温胁迫下显著差异表达的特异基因,其中27个基因上调表达,3个基因下调表达。RT-qPCR证实BnAOC和BnOPR基因均为显著上调基因,BnAOC编码丙二烯氧化物环化酶,属于亲水蛋白,分布在叶绿体上,包含一个Allene-ox-cyc结构域;BnOPR编码12-氧-植物二烯酸还原酶,属于亲水蛋白,主要分布在细胞质,包含一个OYE-like-FMN结构域。不同抗寒性冬油菜的BnAOC和BnOPR基因的碱基均有有义突变的发生,但碱基总数保持不变。
Under freezing stress,orga nisms can change the co mposition and proportion of fatty acids in the membrane system to adapt to cold environment.Linolenic acid and its metabolic intermediates are an important part of fatty acid metabolism in rapeseed.Two varieties,NS(strong cold resistance)and NF(freezing sensitive),were used as test materials,to explore the molecular regulating mechanism of freezing response in Brassica napus.The results showed that the linolenic acid metabolic was significantly enriched pathway at control and-4℃for 12 h,and 30 specific genes were identified under only low-temperature stress between the two cultivars,of which 27 genes were up-regulated and 3 genes were down-regulated.Both BnAOC and BnOPR genes were significantly up-regulated genes as confirmed by RT-qPCR.BnAOC encodes allene oxide cyclase,which is a hydrophilic protein distributed in the chloroplast and contains an Allene-ox-cyc domain.BnOPR encodes 12-oxophytodienoic acid reductase,a hydrophilic protein,mainly distributed in the cytoplasm,containing an OYE-like-FMN domain.There were sense mutations in the bases of BnAOC and BnOPR genes of winter rapeseed with different cold tolerance,but the total number of bases remained unchanged.
作者
李国斌
党林学
郑国强
董小云
魏家萍
崔俊美
李辉
王莹
田海燕
刘自刚
Li Guobing;Dang Linxue;Zheng Guoqiang;Dong Xiaoyun;Wei Jiaping;Cui Junmei;Li Hui;WangYing;Tian Haiyan;Liu Zigang(Promotion Center of Agricultural Technology in Zhuanglang County,Pingliang,744600;State Key Laboratory of Aridland Crop Science,College of Agronomy,Gansu Agricultural University,Lanzhou,730070)
出处
《分子植物育种》
北大核心
2025年第9期2866-2876,共11页
Molecular Plant Breeding
基金
甘肃省教育厅产业支撑计划项目(2021CYZC-46)
甘肃省教育厅青年博士基金项目(2021QB-035)
省部共建干旱生境作物学国家重点实验室主任基金(GSCS-2021-Z01)
中央引导地方科技专项(ZCYD2020-2-3)
西藏农科院农科所统筹基金项目(2021NKS-TCJJ-001)
甘肃省科技重大专项(22ZD6NA009)共同资助。
