摘要
为研究植物生长调节剂对烟草幼苗多酚代谢的影响,以烟草‘NC82’为试材,测定不同浓度水杨酸(salicylic acid,SA)、亚精胺(spermidine,Spd)、茉莉酸甲酯(methyl jasmonate,MeJA)处理下,各处理时间烟草幼苗多酚含量及其合成关键酶苯丙氨酸解氨酶(PAL)活性,并对PAL活性与多酚含量进行相关性分析。结果表明,新鲜烟叶中多酚含量较高的前3种分别是绿原酸、隐绿原酸、新绿原酸;3种植物生长调节剂对烟株多酚含量和PAL活性均有影响,0.25 mmol/L SA及0.1 mmol/L MeJA处理能增加烟草幼苗总多酚含量及提高PAL活性;Spd处理则抑制多酚合成及PAL活性。SA、Spd、MeJA处理下PAL活性与多酚含量整体呈正相关。研究结果可为理解烟草多酚代谢与植物激素之间的关系以及进一步研究植物生长调节剂在提高烟叶品质和抗逆性中的应用提供一定的参考。
In order to study the effect of plant growth regulators on polyphenol metabolism of tobacco seedlings,tobacco'NC82'was used as test material to determine the polyphenol content and phenylalanine ammonia lyase(PAL)activity of tobacco seedlings treated with different concentrations of salicylic acid(SA),spermidine(Spd)and methyl jasmonate(MeJA),and the correlation between PAL activity and polyphenol content was analyzed.The results showed that the first three kinds of polyphenols in fresh tobacco leaves were chlorogenic acid,cryptochlorogenic acid and neochlorogenic acid;these plant growth regulators had effects on polyphenol content and PAL activity of tobacco leaves,0.25 mmol/L SA and 0.1 mmol/L MeJA treatment could increase the content of total polyphenols and PAL activity of tobacco seedlings whether Spd treatment inhibited polyphenols synthesis and PAL activity.PAL activity was positively correlated with polyphenol content under the treatment of SA,Spd and MeJA.The results of this experiment may promote the comprehension between the metabolism of tobacco polyphenols and the effects of plant hormones,while provide a reference to the further study of the application of plant growth regulators in improving the quality and stress resistance of tobacco leaves.
作者
翟雪琴
李显波
郭娟
龙小富
杨倩
刘国琴
彭剑涛
Zhai Xueqin;Li Xianbo;Guo Juan;Long Xiaofu;Yang Qian;Liu Guoqin;Peng Jiantao(College of Life Sciences,Guizhou University,Guiyang,550025;Guizhou Key Laboratory for Tobacco Quality Research,College of Tobacco Science,Guizhou University,Guiyang,550025)
出处
《分子植物育种》
北大核心
2025年第8期2722-2730,共9页
Molecular Plant Breeding
基金
国家自然科学基金项目(32060420)
中国烟草总公司贵州省公司科技项目(201913)共同资助。
关键词
多酚
水杨酸
亚精胺
茉莉酸甲酯
苯丙氨酸解氨酶
Polyphenols
Salicylic acid
Spermidine
Methyl jasmonate
Phenylalanine ammonia lyase
