摘要
采用超声辅助低共熔溶剂(DES)法提取厚朴花多糖。以厚朴花多糖得率为指标,通过单因素试验、Plackett-Burman试验和响应面试验优化提取工艺条件,并对其进行结构表征,同时利用力竭运动诱导的动物氧化应激模型评价厚朴花多糖的体内抗氧化活性。结果显示:最优提取条件为液料比40∶1 (mL/g)、超声时间20 min、超声功率140 W、尿素与氯化胆碱摩尔比2.3∶1、DES含水量65%、超声温度51℃,在此条件下,厚朴花多糖得率达到(13.72±0.35)%。厚朴花多糖提取物具有三螺旋构象,在紫外吸收光谱中未有明显的蛋白质、核苷酸等杂质吸收峰,在红外吸收光谱中具有多糖典型特征峰。动物试验结果表明,厚朴花多糖可明显提高小鼠骨骼肌中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(GAT)的活性,有助于改善力竭运动后机体的氧化应激水平,具有较好的体内抗氧化作用。
The polysaccharides from Magnolia oficinalis flower were extracted by the ultrasonic-assisted deep eutectic solvent(DES)method.Taking the yield of Magnolia officinalis flower polysaccharides as the index,the extraction process conditions were optimized through single-factor tests,Plackett-Burman test and response surface test,and the structure of the polysaccharides was characterized.Meanwhile,the in vivo antioxidant activity of the Magnolia officinalis flower polysaccharides was evaluated by using the animal oxidative stress model induced by exhaustive exercise.The results showed that the optimal extraction conditions were liquid-to-solid ratio 40:1(mL/g),ultrasonic time 20 min,ultrasonic power 140 W,molar ratio of urea to choline chloride 2.3:1,moisture content of DES 65%,and ultrasonic temperature 51 C.Under these conditions,the yield of Magnolia officinalis flower polysaccharides reached(13.72±0.35)%.The polysaccharide extract from Magnolia officinalis flower had a triple-helical conformation.There were no obvious absorption peaks of impurities such as proteins and nucleotides in the UV spectrum,and there were typical characteristic peaks of polysaccharides in the FT-IR spectrum.The results of animal experiments indicated that the Magnolia officinalis flower polysaccharides could significantly increase the activities of superoxide dismutase(SOD),glutathione peroxidase(GSHPx)and catalase(CAT)in the skeletal muscles of mice,which was helpful to improve the oxidative stress level of the body after exhaustive exercise,and had a good in vivo antioxidant effect.
作者
岳艳丽
黄苑玲
YUE Yanli;HUANG Yuanling(Guangzhou City University of Technology,Guangzhou 510000,Guangdong,China)
出处
《粮食与油脂》
北大核心
2025年第6期94-101,共8页
Cereals & Oils
基金
教育部产学合作协同育人项目(2411014251)。
关键词
厚朴花多糖
低共熔溶剂
提取工艺
力竭运动
氧化应激
Magnolia officinalis flower polysaccharide
deep eutectic solvent
extraction process
exhaustive exercise
oxidative stress
